Bi-directional flow assay device with reagent bridge
AssigneeIDEXX Laboratories, Inc.
InventorsScott Marshall Clark, Michael Raymond Kepron
Life of this patent
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Abstract
A device for conducting an assay to determine the presence or amount of an analyte in a fluid sample. The device includes a flow path that facilitates fluidic flow by capillary action. The flow path has a first fluid permeable region, a second fluid permeable region, a barrier that inhibits fluidic flow between the first and second regions, and a fluidic bridge. The bridge includes a fluid permeable material overlapping at least a portion of each of the first region, the barrier and the second region.
Claims28
A device for conducting an assay to determine the presence or amount of an analyte in a fluid sample, the device comprising a flow path that facilitates fluidic flow by capillary action n, wherein the flow path comprises:
- (a) a first fluid permeable region for sample entry;
- (b) a second fluid permeable region;
- (c) a substantially fluid impermeable barrier comprising a hydrophobic spacer between the first and second regions;
- and
- (d) a fluidic bridge comprising a fluid permeable material overlapping at least a portion of each of the first region, the barrier and the second region, for providing fluid communication between the first and second regions, wherein the bridge further comprises a detection zone having an immobilized capture reagent.
A device for conducting an assay to determine the presence or amount of an analyte in a fluid sample, the device comprising a flow path that facilitates fluidic flow by capillary action, comprising:
- (a) a single piece of matrix material comprising a first fluid permeable region for sample entry;
- a second fluid permeable region;
- and a barrier that interrupts fluid communication between the first and second regions comprising an area of the matrix material that has been treated to render the area substantially impermeable to liquid, and
- (b) a fluidic bridge comprising a fluid permeable material overlapping at least a portion of each of the barrier, the first region, and the second region, wherein the bridge provides fluid communication between the first and second regions.
A method for producing a device for conducting an assay to determine the presence or amount of an analyte in a fluid sample, the device comprising a flow path that facilitates fluidic flow by capillary action,
- (a) providing a single piece of fluid permeable matrix material and creating a fluid impermeable barrier on the matrix material between a first region and a second region by treating the area with a substance to render the area substantially impermeable to fluid;
- and
- (b) securing a fluidic bridge comprising a fluid permeable material to the matrix material so that the bridge overlaps at least a portion of each of the
- (c) first region, the barrier and the second region, wherein the bridge provides fluid communication between the first and second regions.
A device for conducting an assay to determine the presence or amount of an analyte in a fluid sample, the device comprising a flow path that facilitates fluidic flow by capillary action, comprising:
- (a) a single piece of hydrophobic matrix material that has been treated to have at least two fluid permeable regions separated by an untreated region that acts as a fluidic barrier between the at least two fluid permeable regions, and
- (b) a fluidic bridge comprising a fluid permeable material overlapping at least a portion of each of the at least two fluid permeable regions and the untreated region, wherein the bridge provides fluid communication between the at least two fluid permeable regions.
A device for conducting an assay to determine the presence or amount of an analyte in a fluid sample, the device comprising a flow path that facilitates fluidic flow by capillary action, wherein the flow path comprises:
- (a) a first fluid permeable region for sample entry;
- (b) a second fluid permeable region;
- (c) a substantially fluid impermeable barrier between the first and second regions;
- and
- (d) a fluidic bridge comprising a fluid permeable material overlapping at least a portion of each of the first region, the barrier and the second region, for providing fluid communication between the first and second regions, wherein the bridge further comprises a detection zone having an immobilized capture reagent;
- wherein the first region, the second region and the barrier comprise a single piece of material and an area of the material comprising the barrier has been treated with a substance to render the area impermeable to liquid.
Dependencies are inferred from the claim text.