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US 7,442,551 B2Granted
Lentiviral packaging constructs
AssigneeNovartis AG
InventorsMichael Kaleko, Tianci Luo, Ivan Plavec, Janet Lynn Douglas
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Abstract
The present invention provides novel lentiviral packaging constructs that are useful for the establishment of stable packaging cell lines and producer cell lines. In particular, the present invention provides novel packaging cell lines that are capable of constitutively expressing high levels of lentiviral proteins.
Claims17
A method for producing a recombinant lentiviral vector particle preparation comprising the steps of:
- transfecting a packaging cell line comprising a packaging construct comprising a sequence encoding a lentiviral protease containing a substitution where T is replaced with S in the DTGAD motif (SEQ ID NO:
- 1) and a deletion in a lentiviral packaging signal sequence with a lentiviral vector,
- propagating the cell line in a suitable culture medium and
- obtaining a recombinant lentiviral vector particles preparation from said culture medium.
1 more element
Dependencies are inferred from the claim text.