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AssigneeNovartis Vaccines and Diagnostics, Inc.
InventorsPaul A. Luciw, Dino Dina, Kathelyn Steimer, Ray Sanchez Pescador, Carlos George-Nascimento, Deborah Parkes, Rob Hallewell, Philip J. Barr, Martha Truett
Estimating the term…
Polynucleotide sequences are provided for the diagnosis of the presence of retroviral infection in a human host associated with lymphadenopathy syndrome and/or acquired immune deficiency syndrome, for expression of polypeptides and use of the polypeptides to prepare antibodies, where both the polypeptides and antibodies may be employed as diagnostic reagents or in therapy, e.g., vaccines and passive immunization. The sequences provide detection of the viral infectious agents associated with the indicated syndromes and can be used for expression of antigenic polypeptides.
In a method for producing an expression product which comprises growing a unicellular microorganism host in a nutrient medium, wherein the unicellular microorganism host comprises a DNA construct having transcriptional and translational initiation and termination regulatory signals functional in the host and a DNA sequence having an open reading frame and under the regulatory control of the signals, whereby said expression product is produced, the improvement comprising:
In a method for producing an expression product which comprises growing mammalian host cells comprising a DNA construct comprising transcriptional and translational initiation and termination regulatory signals functional in the host cells and a DNA sequence, said sequence having an open reading frame and an initiation codon at its 5′ terminus and under the transcriptional and translational control of said regulatory signals, whereby a polypeptide encoded by said sequence is expressed;
Dependencies are inferred from the claim text.