Imaging regulated protein-protein interactions in cells and living animals by enhanced luciferase protein fragment complementation
AssigneeWashington University
InventorsDavid Piwnica-Worms, Kathryn Luker, Gary Luker
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Abstract
An enhanced firefly luciferase protein fragment complementation assay method is described that produces a robust and broadly applicable bioluminescence signal and demonstrates both modification-independent and phosphorylation-dependent protein interactions in intact living human cells and animals useful as a diagnostic and testing tool in living biologic systems in research and in assays.
Claims7
An isolated, characterized and functional complementation system comprising a pair of fusion proteins, each protein fused to one luciferase fragment of a pair of firefly luciferase fragments, the pair commonly having a background bioluminescence when in proximity to a protein fused to the other respective luciferase fragment of the pair such that reconstitution of the pair provides a bioluminescent emitting moiety, wherein the protein pair comprises a polypeptide encoded by SEQ. ID. NO. 1 and a polypeptide encoded by SEQ. ID. NO. 2.
Dependencies are inferred from the claim text.