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US 7,442,517 B2Granted
Method for the detection of Salmonella enterica serovar Enteritidis
AssigneeLawrence Livermore National Security, LLC
InventorsPeter G. Agron, Gary L. Andersen, Richard L. Walker
Life of this patent
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Abstract
Described herein is the identification of a novel Salmonella enterica serovar Enteritidis locus that serves as a marker for DNA-based identification of this bacterium. In addition, three primer pairs derived from this locus that may be used in a nucleotide detection method to detect the presence of the bacterium are also disclosed herein.
Claims11
A method of indicating the presence of Salmonella enterica serovar enteritidis in a sample comprising the steps of:
- providing the sample, wherein the sample contains or does not contain a target DNA sequence SEQ ID NO:
- 1, or a target DNA sequence SEQ ID NO:
- 2, or a target DNA sequence SEQ ID NO:
- 3, or a target DNA sequence SEQ ID NO:
- 4;
- adding primers to the sample, wherein said primers bind to said target DNA sequence SEQ ID NO:
- 1, or target DNA sequence SEQ ID NO:
- 2, or target DNA sequence SEQ ID NO:
- 3, or target DNA sequence SEQ ID NO:
- 4;
- amplifying said target DNA sequence SEQ ID NO:
- 1, or target DNA sequence SEQ ID NO:
- 2, or target DNA sequence SEQ ID NO:
- 3, or target DNA sequence SEQ ID NO:
- 4 that include said bound primers;
- and
- detecting the existence of said target DNA sequence SEQ ID NO:
- 1, or target DNA sequence SEQ ID NO:
- 2, or target DNA sequence SEQ ID NO:
- 3, or target DNA sequence SEQ ID NO:
- 4 by a nucleotide detection method, wherein the existence of said target DNA sequence SEQ ID NO:
- 1, or target DNA sequence SEQ ID NO:
- 2, or target DNA sequence SEQ ID NO:
- 3, or target DNA sequence SEQ ID NO:
- 4 indicates the presence of Salmonella enterica serovar enteritidis in the sample.
22 more elements
A method of indicating the presence of Salmonella enterica serovar enteritidis in a sample comprising the steps of:
- providing the sample, wherein the sample contains or does not contain a target DNA sequence SEQ ID NO:
- 1, or a target DNA sequence SEQ ID NO:
- 2, or a target DNA sequence SEQ ID NO:
- 3;
- adding a primer pair to the sample, wherein said primer pair binds to said target DNA sequence SEQ ID NO:
- 1, or said target DNA sequence SEQ ID NO:
- 2, or said target DNA sequence SEQ ID NO:
- 3,
- said primer pair has the sequence of SEQ ID NO:
- 5 and SEQ ID NO:8 or SEQ ID NO:
- 6 and SEQ ID NO:9 or SEQ ID NO:
- 7 and SEQ ID NO:10;
- amplifying said target DNA sequence SEQ ID NO:
- 1, or said target DNA sequence SEQ ID NO:
- 2, or said target DNA sequence SEQ ID NO:
- 3 that include said bound primer pair;
- and
- detecting the existence of said target DNA sequence SEQ ID NO:
- 1, or said target DNA sequence SEQ ID NO:
- 2, or said target DNA sequence SEQ ID NO:
- 3, wherein the existence of said target DNA sequence SEQ ID NO:
- 1, or said target DNA sequence SEQ ID NO:
- 2, or said target DNA sequence SEQ ID NO:
- 3 indicates the presence of Salmonella enterica serovar enteritidis in the sample.
21 more elements
A method of indicating the presence of Salmonella enterica serovar enteritidis in a sample comprising the steps of:
- providing the sample, wherein the sample contains or does not contain a target DNA sequence SEQ ID NO:
- 1, or a target DNA sequence SEQ ID NO:
- 2, or a target DNA sequence SEQ ID NO:
- 3, or a target DNA sequence SEQ ID NO:
- 4;
- adding a primer pair to the sample, wherein said primer pair bind to said target DNA sequence SEQ ID NO:
- 1, or said target DNA sequence SEQ ID NO:
- 2, or said target DNA sequence SEQ ID NO:
- 3, or said target DNA sequence SEQ ID NO:
- 4;
- said primer pair has the sequence of SEQ ID NO:
- 5 and SEQ ID NO:8 or SEQ ID NO:
- 6 and SEQ ID NO:9 or SEQ ID NO:
- 7 and SEQ ID NO:10;
- amplifying said target DNA sequence SEQ ID NO:
- 1, or said target DNA sequence SEQ ID NO:
- 2, or said target DNA sequence SEQ ID NO:
- 3, or said target DNA sequence SEQ ID NO:
- 4;
- and
- detecting the existence of said target DNA sequence SEQ ID NO:
- 1, or said target DNA sequence SEQ ID NO:
- 2, or said target DNA sequence SEQ ID NO:
- 3, or said target DNA sequence SEQ ID NO:
- 4, by a nucleotide detection method, wherein the existence of said target DNA sequence SEQ ID NO:
- 1, or said target DNA sequence SEQ ID NO:
- 2, or said target DNA sequence SEQ ID NO:
- 3, or said target DNA sequence SEQ ID NO:
- 4 indicates the presence of Salmonella enterica serovar enteritidis in the sample.
26 more elements
Dependencies are inferred from the claim text.