Antibody specific to central nervous system tau protein
AssigneesMitsubishi Chemical Corporation, Mitsubishi Kagaku Iatron, Inc.
InventorsHideto Ohno, Koichi Ishiguro, Masaki Imagawa
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Abstract
The present invention provides an antibody which specifically recognizes a CNS tau protein but not a peripheral tau protein. More specifically, the present invention provides an antibody obtainable by using a polypeptide comprising an amino acid sequence of a connective portion between the amino acid sequence encoded by Exon 4 of a gene encoding a tau protein and the amino acid sequence encoded by Exon 5 thereof as an epitope specific to the isoform of tau protein predominantly existing in central nervous tissues. The present invention further provides a method of detecting Alzheimer's disease and a reagent kit using the antibody.
Claims12
An isolated antibody specific to a central nervous system (CNS) tau protein, wherein the antibody specifically recognizes a CNS tau protein but not a peripheral tau protein, wherein the antibody is obtained by immunizing an animal with a polypeptide comprising an amino acid sequence specific to the CNS tau protein consisting of amino acid residues 124-125 of SEQ ID NO:
- 1 and wherein the antibody specifically recognizes the amino acid sequence specific to CNS tau protein as contained in a polypeptide consisting of amino acid residues selected from the group consisting of 121-128 of SEQ ID NO:
- 1, 118-131 of SEQ ID NO:
- 1, and 121-129 of SEQ ID NO:
- 1.
A method of producing an antibody specific to a CNS tau protein comprising:
- immunizing an animal with a polypeptide containing an amino acid sequence specific to a CNS tau protein as an antigen, wherein the amino acid sequence specific to a CNS tau protein consists of amino acid residues 124-125 of SEQ ID NO:
- 1;
- analyzing reactivity of a resulting antibody with the CNS tau protein and a peripheral tau protein;
- and
- selecting an antibody having reactivity specific to the CNS tau protein, wherein the antibody specifically recognizes the amino acid sequence specific to CNS tau protein as contained in a polypeptide consisting of amino acid residues selected from the group consisting of 121-128 of SEQ ID NO:
- 1, 118-131 of SEQ ID NO:
- 1, and 121-129 of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.