Method of screening for agents that modulate a homer signaling pathway
AssigneeThe Johns Hopkins University
InventorPaul F. Worley
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Abstract
The invention features a method of identifying, evaluating and screening for compounds or agents for the treatment of disorders involving the Homer signaling pathway in the modulation of immunosupression and neuroprotection. The method includes evaluating the ability of agents to modulate Homer protein activity, Homer protein/immunophilin-peptidylproline cis-trans isomerase interaction, and/or Homer protein/proline-type Homer ligand consensus sequence interaction to identify agents for such treatment. The invention also discloses treatment modalities involving agents identified by such methods.
Claims30
A method of screening for activity of modulating agents of a Homer signaling pathway comprising:
- i) contacting at least one protein or peptide having a proline-type Homer ligand consensus sequence in the presence of a peptidylproline cis-trans isomerase (PPIase) inhibitor with at least one test agent for a sufficient time to allow the components to interact;
- ii) contacting the components of step (i) with a Homer protein;
- iii) determining whether binding between the at least one protein or peptide and Homer protein is increased or decreased as compared to the binding in the absence of the test agent, wherein increased or decreased binding between the at least one protein or peptide and Homer protein is indicative of a modulating agent for a Homer signaling pathway.
Dependencies are inferred from the claim text.