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US 7,442,505 B2Granted
Compositions and methods for detecting human herpesviruses
AssigneeBoard of Regents of the University of Nebraska
InventorsRonald Rubocki, Catherine Gebhart
Life of this patent
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Abstract
The present invention relates to primer compositions and methods for identifying and distinguishing CMV, EBV-A/B, HSV-1/2, HHV-6 and VZV herpesviruses in a sample.
Claims3
A composition for amplifying a DNA polymerase nucleic acid of a select human herpesvirus comprising three or more primer sets of:
- SEQ ID NO:6 and SEQ ID NO:7;
- specific for amplification of CMV;
- SEQ ID NO:8 and SEQ ID NO:9;
- specific for amplification of HSV;
- SEQ ID NO:10 and SEQ ID NO:11;
- specific for amplification of EBV;
- SEQ ID NO:12 and SEQ ID NO:13;
- specific for amplification of VZV or
- SEQ ID NO:14 and SEQ ID NO:15;
- specific for amplification of HHV, each of said primers generating amplicons of five distinct sizes which are indicative of the type of herpesvirus being amplified.
7 more elements
Dependencies are inferred from the claim text.