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US 7,442,501 B2Granted
SQV nucleic acids and polypeptides
AssigneeMassachusetts Institute of Technology
InventorsH. Robert Horvitz, Ho Yon Hwang
Life of this patent
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Abstract
The invention generally features sqv nucleic acid and polypeptide molecules associated with connective tissue diseases, progeroid disorders, and aging, and methods for isolating such molecules.
Claims8
A method of identifying a compound that modulates a glycosaminoglycan biosynthetic biological activity, said method comprising the steps of:
- (a) providing a first cell comprising a wild-type sqv nucleic acid molecule;
- (b) providing a second cell comprising a sqv-1, sqv-2, sqv-4, sqv-5, or sqv-6 mutant nucleic acid molecule;
- (c) contacting said first cell with a candidate compound;
- (d) contacting said second cell with said candidate compound;
- and
- (e) detecting, in a protein extract from said first or second cell, an alteration of an enzymatic activity that contributes to the production of a glycosaminoglycan in said first cell when compared to said second cell, wherein said alteration identifies said compound as modulating a glycosaminoglycan biosynthetic biological activity.
3 more elements
A method of identifying a compound that modulates a glycosaminoglycan biosynthetic biological activity, said method comprising the steps of:
- (a) providing a first nematode comprising a wild-type sqv nucleic acid molecule;
- (b) providing a second nematode comprising a sqv-1, sqv-2, sqv-4, sqv-5, or sqv-6 mutant nucleic acid molecule;
- (c) contacting said first nematode with a candidate compound;
- (d) contacting said second nematode with said candidate compound;
- and
- (e) detecting, in a protein extract from said first or second nematode, an alteration of an enzymatic activity that contributes to the production of a glycosaminoglycan in said first nematode when compared to said second nematode, wherein said alteration identifies said compound as modulating a glycosaminoglycan biosynthetic biological activity.
3 more elements
Dependencies are inferred from the claim text.