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AssigneeRepublic of National Fisheries Research and Development Institute
InventorsYoung Ok Kim, Han Woo Kim, Jeong Ho Lee, Kyung Kil Kim, Jong Yun Lee, In Soo Kong
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The present invention relates to phytase enzyme, a gene encoding the enzyme, and a Citrobacter sp. producing the enzyme. Particularly, the present invention relates to the phytase enzyme produced from Citrobacter sp. having (a) molecular weight of 47 kDa, (b) optimal pH of 3.5-4.5, (c) optimal temperature of 45-55° C., (d) a substrates phytate, p-nitrophenyl phosphate, tetrasodium pyrophosphate, ATP or ADP, (e) Michaelis constant of 0.3-0.5 mM utilizing phytate as substrate, and (f) high resistance to protease such as pepsin, trypsin, papain, elastase or pancreatin. The present invention also relates to the gene coding the phytase enzyme and the Citrobacter braakii producing the enzyme. The phytase enzyme and the Citrobacter braakii producing the enzyme of the present invention can be used in manufacturing a feed of monogastrics as feed additive and in recovering a specific decomposition product of phytate at low price.
An isolated or recombinant protein having phytase activity, said protein consisting of the amino acid sequence of SEQ ID NO:
An isolated gene encoding a protein having phytase activity, said protein comprising the amino acid residues 23-433 of SEQ ID NO:
Dependencies are inferred from the claim text.