Loading patent…
AssigneeMount Sinai School of Medicine of New York University
InventorsAdolfo Garcia-Sastre, Peter Palese
Estimating the term…
This invention relates to genetically engineered Newcastle disease viruses and viral vectors which express heterologous genes or mutated Newcastle disease viral genes or a combination of viral genes derived from different strains of Newcastle disease virus. The invention relates to the construction and use of recombinant negative strand NDV viral RNA templates which may be used with viral RNA-directed RNA polymerase to express heterologous gene products in appropriate host cells and/or to rescue the heterologous gene in virus particles. In a specific embodiment of the invention, the heterologous gene product is a peptide or protein derived from the genome of a human immunodeficiency virus. The RNA templates of the present invention may be prepared by transcription of appropriate DNA sequences using any DNA-directed RNA polymerase such as bacteriophage T7, T3, SP6 polymerase, or eukaryotic polymerase I.
A recombinant RNA molecule comprising a binding site specific for an RNA polymerase of a Newcastle disease virus and signals required for NDV mediated replication and transcription, operatively linked to a heterologous RNA sequence, wherein the signals required for NDV mediated replication and transcription is contained in the 3′ and 5′ -noncoding flanking regions of the NDV viral RNA genome.
A recombinant RNA molecule comprising a binding site for an RNA polymerase of a Newcastle disease virus and signals required for NDV mediated replication and transcription, operatively linked to a Newcastle disease viral gene;
Dependencies are inferred from the claim text.