Attenuated circovirus
AssigneeThe University of Melbourne
InventorsGlenn Francis Browning, Kelly Ann Tivendale, Peter Christopher Scott, Hayley Kay Brown, Brendan Scott Crabb, Michelle Alma Peters
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Abstract
An isolated attenuated circovirus having a mutation in viral nucleic acid encoding viral protein 2 (VP2). The attenuated circovirus is particularly suitable for use in conferring immunity in an animal, particularly birds.
Claims37
A method for imparting or conferring immunity to circovirus infection in an animal comprising administering to the animal an effective amount of a circovirus vaccine, wherein the vaccine comprises an isolated nucleic acid molecule derived or obtained from a circovirus genome, the nucleic acid molecule including at least a portion of a coding region for viral protein 2 (VP2) of at least 20 nucleotides in length having a mutation therein, wherein the mutation is present in a nucleic acid region encoding amino acid residue 87, 95, 97, 101, 102, 103, 129, 131, 150, 151, 152, or 161 to 170 as set out in SEQ ID NO:
- 2;
- or the vaccine comprises an isolated attenuated circovirus capable of cellular replication and having a mutation in viral nucleic acid encoding viral protein 2 (VP2), wherein the mutation is present in a nucleic acid region encoding amino acid residue 87, 95, 97, 101, 102, 103, 129, 131, 150, 151, 152, or 161 to 170 as set out in SEQ ID NO:
- 2.
A method of producing a circovirus vaccine comprising:
- (a) inoculating an isolated nucleic acid molecule derived or obtained from a circovirus genome into the yolk sac of an embryonated egg, wherein the nucleic acid molecule includes at least a portion of a coding region for viral protein 2 (VP2) of at least 20 nucleotides in length having a mutation therein, wherein the mutation is present in a nucleic acid region encoding amino acid residue 87, 95, 97, 101. 102, 103, 129, 131, 150, 151, 152, or 161 to 170 as set out in SEQ ID NO:
- 2;
- (b) allowing circovirus to replicate from the isolated nucleic acid;
- and
- (c) harvesting the circovirus from the egg.
An isolated attenuated circovirus capable of cellular replication and having a mutation in viral nucleic acid encoding viral protein 2 (VP2), wherein the mutation is present in a nucleic acid region encoding amino acid residue 87, 95, 97, 101, 102, 103, 129, 131, 150, 151, 152, or 161 to 170 as set out in SEQ ID NO:
- 2.
An isolated nucleic acid molecule derived or obtained from a circovirus genome, the nucleic acid molecule including at least a portion of a coding region for viral protein 2 (VP2) of at least 20 nucleotides in length having a mutation therein, wherein the mutation is present in a nucleic acid region encoding amino acid residue 87, 95, 97, 101, 102, 103, 129, 131, 150, 151, 152, or 161 to 170 as set out in SEQ ID NO:2.
Dependencies are inferred from the claim text.