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AssigneeIntel Corporation
InventorsNarayanan Sundararajan, Lei Sun, Yuegang Zhang, Xing Su, Selena Chan, Tae-Woong Koo, Andrew A. Berlin
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Disclosed herein are methods, apparatuses, and systems for performing nucleic acid sequencing reactions and molecular binding reactions in a microfluidic channel. The methods, apparatuses, and systems can include a restriction barrier to restrict movement of a particle to which a nucleic acid is attached. Furthermore, the methods, apparatuses, and systems can include hydrodynamic focusing of a delivery flow. In addition, the methods, apparatuses, and systems can reduce non-specific interaction with a surface of the microfluidic channel by providing a protective flow between the surface and a delivery flow.
An apparatus comprising, a first channel comprising a restriction barrier comprising a first angled wall and a second angled wall positioned relative to the first angled wall to form a first opening at least 1 micron in width or diameter and a second opening less than 10 microns in width or diameter, wherein the first opening has a greater width or diameter than the second opening, further comprising a laser light source and a series of lenses to form a gradient force optical trap operable as optical tweezers configured to capture a particle downstream of the restriction barrier and release it upstream of the restriction barrier, a light source and a Raman detector to detect a single molecule by a surface enhanced Raman spectroscopy, the first channel being in optical communication with the light source and the Raman detector.
A system comprising:
An apparatus comprising a first channel having a restriction barrier within the channel, the restriction barrier comprising a first angled wall and a second angled wall positioned relative to the first angled wall to form a first opening large enough to capture a single particle and a second opening small enough to prevent passage of the particle but large enough to allow passage of a biomolecule, wherein the first opening is at least 100 nm wide and the second opening is less than 10 microns wide, and wherein the first opening has a greater width or diameter than the second opening, further comprising a laser light source and a series of lenses to form a gradient force optical trap operable as optical tweezers configured to capture a particle downstream of the restriction barrier and release it upstream of the restriction barrier,
Dependencies are inferred from the claim text.