Amperometric biosensor in thick film technology
AssigneesAlexander Adlassnig, Julius Schuster
InventorsAlexander Adlassnig, Juliua Schuster
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Abstract
Described is an amperometric biosensor in thick film technology for the detection an/or determination of substances undergoing enzyme catalyzed reactions, especially glucose or lactate, comprising an inert carrier material, at least one transducer layer exhibiting a specific electrical conductivity χ>104 Ω-1cm-1, and at least one bioactive layer with diffusion barrier function containing at least one enzyme specifically reacting with said substance to be measured, said bioactive layer exhibiting a specific electrical conductivity χ<1 Ω-1cm-1 and said bioactive layer further effectively hinders other electroactive substances to reach the transducer layer within the time of measurement.
Claims9
A thick film amperometric biosensor capable of detection and/or determination of a substance undergoing an enzyme-catalyzed reaction comprising:
- an inert carrier material;
- at least one transducer layer exhibiting a specific electrical conductivity χ>104Ω−1cm−1, during use of the biosensor;
- and
- at least one bioactive layer adapted to function as a diffusion barrier during use of the biosensor, the bioactive layer further defined as:
- comprising at least one enzyme capable of specifically reacting with the substance to be detected or determined during use of the biosensor;
- exhibiting a specific electrical conductivity of χ<1Ω−1cm−1 during use of the biosensor;
- and
- capable of effectively hindering electroactive substances, other than any produced by the substance undergoing the enzyme-catalyzed reaction, from reaching the transducer layer during use of the biosensor.
A process for producing a thick film amperometric biosensor capable of detection and/or determination of a substance undergoing an enzyme-catalyzed reaction comprising:
- providing an inert carrier material;
- depositing at least one electrically conducting layer on the inert carrier material;
- and
- depositing at least one bioactive layer capable of functioning as a diffusion barrier during use of the biosensor onto the at least one electrically conducting layer by screen printing using a transducer-free printing ink comprising an enzyme and a biocompatible polymer, this depositing being accomplished without the use of UV-radiation, wherein the bioactive layer is capable of effectively hindering electroactive substances, other than any produced by the substance undergoing the enzyme-catalyzed reaction, from reaching the transducer layer during use of the biosensor.
A printing ink comprising an enzyme, wherein the ink is screen printable without the use of UV-radiation and capable of, in an amperometric biosensor capable of detection and/or determination of a substance undergoing an enzyme-catalyzed reaction, forming at least one bioactive layer, adapted to:
- function as a diffusion barrier during use of the biosensor;
- have a specific electrical conductivity of χ<1 Ω−1cm−1 during use of the biosensor;
- and
- effectively hinder electroactive substances other than any produced by the substance undergoing the enzyme-catalyzed reaction from reaching a transducer layer during use of the biosensor.
A method of detection and/or determination of a substance undergoing an enzyme-catalyzed reaction comprising:
- obtaining a thick film amperometric biosensor capable of detection and/or determination of a substance undergoing an enzyme-catalyzed reaction comprising:
- (a) an inert carrier material;
- (b) at least one transducer layer exhibiting a specific electrical conductivity χ>104 Ω−1cm−1, during use of the biosensor;
- and (c) at least one bioactive layer adapted to function as a diffusion barrier during use during use of the biosensor, the bioactive layer further defined as:
- (i) comprising at least one enzyme capable of specifically reacting with the substance to be detected or determined during use of the biosensor;
- (ii) exhibiting a specific electrical conductivity of χ<1 Ω−1cm−1 during use of the biosensor;
- and (iii) capable of effectively hindering electroactive substances, other than any produced by the substance undergoing the enzyme-catalyzed reaction, from reaching the transducer layer during use of the biosensor;
- obtaining a sample;
- and
- using the biosensor to detect and/or determine a substance in the sample.
Dependencies are inferred from the claim text.