Process and systems for peptide synthesis

A method of synthesizing a peptide, comprising steps of:

• a. providing a composition comprising a peptide fragment, wherein the peptide fragment comprises at least one amino acid residue and includes a base-sensitive, N-terminal protecting group;
• b. removing the base-sensitive, N-terminal protecting group from the peptide fragment using a deprotection reagent comprising a base, whereby an N-terminal functionality on the peptide fragment is deprotected;
• c. removing the base from the composition to a point where the composition would provide a positive test result if subjected to a chloranil test;
• d. causing a reactive peptide fragment having a reactive C-terminus and a base-sensitive N-terminal protecting group to react with the deprotected N-terminal functionality of the peptide fragment under conditions such that the reactive peptide fragment is added to the peptide fragment;
• and
• e. optionally repeating steps (b) through (d) until a desired peptide is obtained.

A method of synthesizing a peptide, comprising steps of:

• a. providing a composition comprising a peptide fragment, wherein the peptide fragment comprises at least one amino acid residue and includes a base-sensitive, N-terminal protecting group;
• b. removing the base-sensitive N-terminal protecting group from the amino acid residue using a deprotection reagent comprising a base, whereby an N-terminal functionality on the peptide fragment is deprotected;
• c. causing a reactive peptide fragment having a reactive C-terminus and a base-sensitive, N-terminal protecting group to react with the deprotected N-terminal functionality of the peptide fragment under conditions such that the reactive peptide fragment is added to the peptide fragment, wherein the composition has a basic pH during the reaction of the reactive peptide fragment and the peptide fragment;
• and
• d. optionally repeating steps (b) and (c) until a desired peptide is obtained.