Modified cell line and method for expansion of NK cell
AssigneeSt. Jude Children's Research Hospital
InventorsDario Campana, Chihaya Imai
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Abstract
The present invention relates to novel methods for preferentially activating and expanding NK cells. The methods utilize the stimulatory effects of IL-15 and CD137 ligand to preferentially expand and activate NK cells in a mixed cell culture comprising NK cells.
Claims3
A modified cell line comprising K562 myeloid leukemia cells that lack major histocompatibility complex I molecules wherein said K562 myeloid leukemia cells are genetically modified to express membrane bound interleukin-15 and co-stimulatory factor ligand CD137L, wherein said modified cell line activates natural killer (NK) cells and fails to activate T lymphocytes.
A method of preferentially expanding natural killer (NK) cells in a mixed cell culture comprising NK cells and T lymphocytes which comprises culturing said mixed cell culture with a modified cell line comprising K562 myeloid leukemia cell that lack major histocompatibility complex I molecules wherein said modified K562 myeloid leukemia cells are genetically modified to express membrane bound interleukin-15 and co-stimulatory factor ligand CD137L, wherein said modified cell line activates natural killer (NK) cells and fails to activate T lymphocytes.
Dependencies are inferred from the claim text.