Microbial production of COQ10
AssigneeDsm IP Assets B.V.
InventorsMarkus Huembelin, Rual Lopez-Ulibarri, John B. Perkins, Ghislain Schyns
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Abstract
The present Invention relates to enzymes involved in the Synthesis of Coenzyme Q-10, i.e., decaprenyl diphosphate (DPP) synthase and 4-hydroxybenzoate polyprenyltransferase, to isolated DNA encoding said enzymes and to methods for the microbial production of Coenzyme Q-10.
Claims12
An isolated DNA comprising
- (1) a nucleotide sequence that encodes decaprenyl diphosphate (DPP) synthase, said nucleotide sequence being selected from the group consisting of
- (a) the DNA sequence of SEQ ID NO:
- 16;
- (b) a DNA sequence which hybridizes under stringent conditions comprising hybridization in a buffer of 40% formamide, 1 M NaCl, 1% sodium dodecyl sulfate (SDS) at 37° C. and at least one wash in 0.2×SSC at a temperature of 60° C. for 20 minutes, to the full length complementary strand of SEQ ID NO:
- 16, and encodes a polypeptide having the activity of DPP synthase;
- and
- (c) a DNA sequence which is at least 90% identical to a DNA which codes for the polypeptide of SEQ ID NO:
- 17, said polypeptide having DPP synthase activity;
- or
- (2) a nucleotide sequence that encodes 4-hydroxybenzoate polyprenyltransferase, said nucleotide sequence being selected from the group consisting of:
- (a′) the DNA sequence of SEQ ID NO:
- 23;
- (b′) a DNA sequence which hybridizes under stringent conditions comprising hybridization in a buffer of 40% formamide, 1 M NaCl, 1% sodium dodecyl sulfate (SDS) at 37° C. and at least one wash in 0.2×SSC at a temperature of 60° C. for 20 minutes, to the full length complementary strand of SEQ ID NO:
- 23, and encodes a polypeptide having the activity of 4-hydroxybenzoate polyprenyltransferase;
- and
- (c′) a DNA sequence which is at least 90% identical to a DNA which codes for the polypeptide of SEQ ID NO:
- 24, said polypeptide having 4-hydroxybenzoate polyprenyltransferase activity.
An isolated polynucleotide comprising the nucleotide sequence of SEQ ID NO:
- 16.
An isolated polynucleotide consisting of the nucleotide sequence of SEQ ID NO:
- 16.
An isolated polynucleotide that encodes the polypeptide sequence of SEQ ID NO:
- 17.
An isolated polynucleotide comprising the nucleotide sequence of SEQ ID NO:
- 23.
An isolated polynucleotide consisting of the nucleotide sequence of SEQ ID NO:
- 23.
An isolated polynucleotide that encodes the polypeptide of SEQ ID NO:
- 24.
Dependencies are inferred from the claim text.