Fluorescent polymeric materials containing lipid soluble rhodamine dyes
AssigneeApplied Biosystems, LLC
InventorsJoe Y. L. Lam, Steven M. Menchen, Ruiming Zou, Scott C. Benson
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Abstract
Fluorescent polymeric materials are disclosed comprising a polymer and one or more lipid soluble rhodamine dyes. The materials are especially useful in the preparation of multicolored microparticles, especially multicolored polystyrene microparticle, for use in the multiplexed analysis of a plurality of analytes in a single sample. When excited by a light source, the materials give off a unique emission based on the nature, concentration and ratio of the dyes therein. Methods of preparing and using said materials are also disclosed.
Claims33
A fluorescent polymeric material comprising a polymeric particle and a lipid-soluble rhodamine dye according to structural formula (I):
- wherein:
- R1 and R8 are each, independently of one another, selected from hydrogen, (C1-C8) alkyl and halo, or one of R1 or R8 is part of an optionally substituted benzo group with R2 or R7, respectively, and the other one of R1 and R8 is selected from hydrogen, (C1-C8) alkyl and halo;
- R2 and R7 are each, independently of one another, selected from hydrogen, (C1-C8) alkyl and halo, or one of R2 or R7 is part of an optionally substituted benzo group with R1 or R8, respectively, and the other one of R2 or R7 is selected from hydrogen, (C1-C8) alkyl and halo, or R2 and R7 are part of optionally substituted 5- or 6-membered rings with R3′ and R6′, respectively;
- R3′ and R6′ are each the same or different (C4-C20) alkyl, or are part of optionally substituted 5- or 6-membered rings with R2 and R7, respectively;
- R3″ and R6″ are each, independently of one another, selected from hydrogen and the same or different (C4-C20) alkyl, or are part of optionally substituted 5- or 6-membered rings with R4 and R4 and R5, respectively;
- R4 and R5 are each, independently of one another, selected from hydrogen, (C1-C8) alkyl, halo, aryl and heteroaryl, or are part of optionally substituted 5-or 6-membered rings with R3″ and R6″, respectively;
- R11 and R15 are each hydrogen or are each F or Cl;
- and
- R12, R13 and R14 are each hydrogen or are each F or Cl,
- and wherein the lipid-soluble rhodamine dye is incorporated into the polymeric particle non-covalently.
Dependencies are inferred from the claim text.