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US 7,432,074 B2Granted
Method for extracting a macrolide from biomatter
AssigneeTEVA Gyógyszergyár Zártkörüen MüködöRészvénytársaság
InventorsVilmos Keri, Lajos Deak, Csaba Szabo
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Abstract
Provided is a method for obtaining a macrolide, especially tacrolimus, from biomatter.
Claims24
A method for obtaining a macrolide comprising the step of extracting macrolide-containing biomatter that is whole fermentation broth with a hydrophobic extraction solvent to obtain a solution of the macrolide in the hydrophobic extraction solvent, wherein the pH of the macrolide-containing biomatter being extracted is between 8 and about 13 and recovering said macrolide from said solution.
A method of obtaining a macrolide from a macrolide-containing biomatter comprising the steps of:
- a) extracting macrolide-containing biomatter that is whole fermentation broth obtained by fermentation of a microorganism selected from the group consisting of Streptomyces tsukubaensis, Streptomyces hygroscopicus, and Streptomyces lividans with iso-butyl acetate wherein the pH of the macrolide-containing biomatter is between 8 and about 13 to obtain a solution of a macrolide in iso-butyl acetate at a temperature between about 2° C. and about 70° C.,
- b) isolating the macrolide-containing solution from the extracted macrolide-containing biomatter,
- c) concentrating the macrolide-containing solution,
- d) purifying the concentrated macrolide-containing solution on a silica gel chromatography column to obtain at least one macrolide-containing fraction that is a macrolide-containing solution,
- e) concentrating at least one macrolide-containing fraction, wherein the macrolide is tacrolimus, and
- (f) recovering said tacrolimus from step (e).
3 more elements
Dependencies are inferred from the claim text.