Methods of controlling proliferation and differentiation of stem and progenitor cells
AssigneesGamida Cell Ltd., Hadasit Medical Research Services and Development Ltd.
InventorsTony Peled, Eitan Fibach, Avi Treves
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Abstract
A method of ex-vivo expanding a population of stem cells, while at the same time inhibiting differentiation of the stem cells. The method comprises ex-vivo providing the stem cells with conditions for cell proliferation and with at least one copper chelator in an amount and for a time period for permitting the stem cells to proliferate and, at the same time, for reducing a capacity of the stem cells to differentiate.
Claims59
An ex-vivo expanded hematopoietic cell population comprising cells having reduced available intracellular copper as compared to ex vivo hematopoietic cells cultured in the absence of transition metal chelator having affinity for copper and wherein said cells are further characterized by increased clonogenic potential, engraftment potential, hematopoietic reconstitution potential and/or proportion of CD34+ cells.
An expanded hematopoietic cell population obtained by ex-vivo culturing hematopoietic cells in a culture medium providing said hematopoietic cells with conditions for cell proliferation, said conditions comprising nutrients and cytokines, and in the presence of a transition metal chelator having affinity for copper, wherein said conditions and said transition metal chelator result in reduced available intracellular copper and increased expansion of clonogenic cells following at least one week in the presence of said nutrients and cytokines and said transition metal chelator having affinity for copper as compared to ex vivo hematopoietic cells cultured for the same time in a presence of said cytokines yet in an absence of said transition metal chelator.
A hematopoietic cell population comprising ex-vivo expanded cells derived from peripheral blood, bone marrow and/or neonatal umbilical cord blood and characterized by reduced available intracellular copper concentration as compared to hematopoietic cells expanded ex-vivo in the absence of a transition metal chelator having affinity for copper and wherein said cells are further characterized by increased clonogenic potential, engraftment potential, hematopoietic reconstitution potential and/or proportion of CD34+ cells.
Dependencies are inferred from the claim text.