Recombinant production of mixtures of antibodies
AssigneeCrucell Holland B.V.
InventorsPatrick H. C. Van Berkel, Ronald H. P. Brus, Ton Logtenberg, Abraham Bout
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Abstract
The invention provides methods for producing mixtures of antibodies from a single host cell clone, wherein, a nucleic acid sequence encoding a light chain and nucleic acid sequences encoding different heavy chains are expressed in a recombinant host cell. The recombinantly produced antibodies in the mixtures according to the invention suitably comprise identical light chains paired to different heavy chains capable of pairing to the light chain, thereby forming functional antigen-binding domains. Mixtures of the recombinantly produced antibodies are also provided by the invention. Such mixtures can be used in a variety of fields.
Claims20
An isolated host cell capable of producing three or more non-identical antibodies, said host cell comprising:
- at least one exogenously introduced nucleic acid sequence encoding an immunoglobulin light chain and exogenously introduced nucleic acid sequences encoding at least three different immunoglobulin heavy chains, wherein said at least three different immunoglobulin heavy chains are capable of pairing with said immunoglobulin light chain to form the three or more non-identical antibodies that bind antigen.
Dependencies are inferred from the claim text.