Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method
AssigneeSigma-Aldrich Co.
InventorsWolfgang Pieken, Andreas Wolter, David P. Sebesta, Michael Leuck, Hallie A. Latham-Timmons, John Pilon, Gregory M. Husar
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Abstract
This invention discloses a novel method for immobilizing molecules to a support. Specifically, this invention discloses a method of immobilizing derivatized biomolecules, such as oligonucleotides, using cycloaddition reactions, such as the Diels-Alder reaction. Included in this invention are the novel immobilized biomolecules that can be prepared according to the method of this invention.
Claims20
A method for immobilizing a molecule on a support comprising the steps of:
- a) derivatizing the molecule with a functional group capable of undergoing a cycloaddition reaction, wherein said derivatization is independent of the synthesis of the molecule;
- and
- b) immobilizing the derivatized molecule by reaction with a derivatized support capable of reacting with said derivatized molecule via a cycloaddition reaction.
A method for immobilizing a molecule on a support comprising the step of reacting a derivatized molecule with a derivatized support capable of reacting with said derivatized molecule via a cycloaddition reaction, wherein said molecule is a diagnostic detector molecule (DDM).
A method for immobilizing a molecule on a support comprising the step of reacting a derivatized molecule with a support capable of reacting with said derivatized molecule via a cycloaddition reaction, wherein said molecule is derivatized with a diene selected from the group consisting of:
- wherein
- Z is a linker selected from the group consisting of:
- wherein
- m, n, o are equal to 0, 1, 2 and
- Y is selected from NH, O, NH(CO)O, NH(CS)O, NH(CO)NH, NH(CO), S—S—S—, Si(OR)3 and SiR2 wherein
- R is selected from alkyl, aryl, substituted alkyl or substituted aryl.
A method for immobilizing a molecule on a support comprising the step of reacting a derivatized molecule with a support capable of reacting with said derivatized molecule via a cycloaddition reaction, wherein said molecule is derivatized with a dienophile selected from the group consisting of:
- wherein
- Z is a linker selected from the group consisting of:
- wherein
- m, n, o are equal to 0, 1, 2 and
- Y is selected from NH, O, NH(CO)O, NH(CS)O, NH(CO)NH, NH(CO), S—S—S—, Si(OR)3 and SiR2 wherein
- R is selected from alkyl, aryl, substituted alkyl or substituted aryl.
A method for immobilizing a molecule on a support comprising the step of reacting said molecule with a support capable of reacting with said molecule via a cycloaddition reaction wherein said support is derivatized with a diene selected from the group consisting of:
- wherein
- Z is a linker selected from the group consisting of:
- wherein
- m, n, o are equal to 0, 1, 2 and
- Y is selected from NH, O, NH(CO)O, NH(CS)O, NH(CO)NH, NH(CO), S—S—S—, Si(OR)3 and SiR2 wherein
- R is selected from alkyl, aryl, substituted alkyl or substituted aryl.
A method for immobilizing a molecule on a support comprising the step of reacting said molecule with a support capable of reacting with said molecule via a cycloaddition reaction wherein said support is derivatized with a dienophile selected from the group consisting of:
- wherein
- Z is a linker selected from the group consisting of:
- wherein
- m, n, o are equal to 0, 1, 2 and
- Y is selected from NH, O, NH(CO)O, NH(CS)O, NH(CO)NH, NH(CO), S—S—S—, Si(OR)3 and SiR2 wherein
- R is selected from alkyl, aryl, substituted alkyl or substituted aryl.
Dependencies are inferred from the claim text.