Genetic analysis and authentication
AssigneeBio Array Solutions Ltd.
InventorsGhazala Hashmi, Michael Seul, Joachim Messing
Life of this patent
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Abstract
This invention provides compositions and methods for genetic testing of an organism and for correlating the results of the genetic testing with a unique marker that unambiguously identifies the organism. The markers may be internal markers, such as for example single nucleotide polymorphisms (SNPs), short tandem repeats (STRs), or other sites within a genomic locus. Alternatively, the markers may be external, such that they are separately added to the genetic sample before testing.
Claims25
A method for analyzing a target nucleic acid sequence in a patient genomic sample and for identifying said patient providing the sample, said method comprising the following steps:
- (a) providing a first set of oligonucleotides, wherein members of the first set have different sequences and said members are attached to beads which are associated with an optically distinguishable characteristic that identifies the sequences of the oligonucleotides attached thereto, the first set of oligonucleotides for identifying target nucleic acid sequences in the patient sample;
- (b) a second set of oligonucleotide wherein members of the second set have different sequences and said members are attached to beads which are associated with an optically distinguishable characteristic that identities the sequences of the oligonucleotides attached thereto, the second set of oligonucleotides for identifying a number of marker sequences in the patient genomic sample, said marker sequences each including at least one polymorphic marker;
- (c) contacting the target sequence and a plurality of marker sequences to said first and said second set of oligonucleotides under conditions permitting annealing of the first set of oligonucleotides to the target sequence or annealing of the second set of oligonucleotides to the marker sequences, or said annealing followed by elongation of the annealed oligonucleotides;
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- (d) detecting the annealing or annealing followed by elongation between the oligonucleotides of the first set to the target sequence and the oligonucleotides of the second set with the marker sequences, said method thereby providing the analysis of the target sequence and the identification of said patient.
A method for analyzing a target nucleic acid sequence in a patient genomic sample and for identifying said patient providing the sample, said method comprising the following steps:
- (a) providing a first set of oligonucleotides wherein members of the first set have different sequences and sad members are attached to beads which are associated with an optically distinguishable characteristic that identifies the sequences of the oligonucleotides attached thereto, the first set of oligonucleotides for identifying target nucleic acid sequences;
- (b) contacting said first set of oligonucleotides with a solution containing the target nucleic acid sequence under conditions permitting annealing of the first set of oligonucleotides with the target nucleic acid sequence or said annealing followed by elongation of the annealed oligonucleotides;
- (c) labeling the solution with a molecular label that uniquely identifies said target solution, such the patient identity is determined by interrogating said label, wherein the label may be added to said sample before said target solution is contacted with the first set of oligonucleotides, or afterwards, or concurrently therewith;
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- (d) detecting said annealing or said annealing followed by elongation of said oligonucleotides and correlating the optically distinguishable characteristic with the oligonucleotides which annealed or which were elongated in order to identify said oligonucleotides and thereby identify the patient providing the sample.
Dependencies are inferred from the claim text.