Hepatitis-B viral variants with reduced susceptibility to nucleoside analogs and uses thereof
AssigneeInnogenetics N.V.
InventorMithat Bozdayi
Life of this patent
Estimating the term…
Abstract
The present invention relates generally to the field of Hepatitis B variants exhibiting a reduced sensitivity to nucleoside analogues, both in vivo and in vitro. More in particular, reverse transcriptase mutant rtA181S is provided. Present invention provides assays and methods for detecting such variant, which assays are useful in monitoring anti-viral therapeutic regimes and adjusting patient therapy. A diagnostic kit for detecting the presence of an HBV variant in a biological sample has also been described.
Claims8
A method for detecting the presence of an HBV variant in a biological sample, said HBV variant comprising at least one nucleotide mutation in the DNA polymerase gene at codon 181 which results in an alanine to serine amino acid substitution rtA181S, said method comprising the step of detecting the presence of a HBV polynucleic acid in a biological sample, said HBV polynucleic acid comprising a nucleotide mutation that results in an amino acid substitution rtA181S of the DNA polymerase gene in the HBV variant, or a fragment of said HBV polynucleic acid comprising said nucleotide mutation.
A method for detecting the resistance of an HBV virus present in a biological sample to an antiviral drug, said HBV variant comprising at least one nucleotide mutation in the DNA polymerase gene at codon 181 which results in an alanine to serine amino acid substitution rtA181S, said method comprising the step of detecting the presence of a HBV polynucleic acid comprising a nucleotide mutation that results in an amino acid substitution rtA181S of the DNA polymerase gene in the HBV variant, or a fragment of said HBV polynucleic acid comprising said nucleotide mutation, wherein the presence of said HBV polynucleic acid indicates resistance of an HBV virus present in said biological sample to an antiviral drug.
Dependencies are inferred from the claim text.