Expression of human milk proteins in transgenic plants
AssigneeVentria Bioscience
InventorsNing Huang, Raymond L. Rodriguez, Frank E. Hagie
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Abstract
The invention is directed to seed and seed extract compositions containing levels of a human milk protein between 3-40% or higher of the total protein weight of the soluble protein extractable from the seed. Also disclosed is a method of producing the seed with high levels of extractable human milk protein. The method includes transforming a monocotyledonous plant with a chimeric gene having a protein-coding sequence encoding a protein normally present in human milk under the control of a seed maturation-specific promoter. The method may further includes a leader DNA sequence encoding a monocot seed-specific transit sequence capable to target a linked milk protein to a storage body.
Claims17
A method of producing a recombinant milk protein in monocot plant seeds comprising the steps:
- (a) obtaining a monocot plant stably transformed with a chimeric gene having (i) a seed maturation-specific promoter, (ii) operably linked to said promoter, a leader DNA sequence encoding a monocot seed-specific transit sequence capable of targeting a linked polypeptide to an endosperm-cell organelle, wherein the promoter operably linked to the leader sequence comprises either SEQ ID NO:13 or SEQ ID NO:14 and (iii) a protein-coding sequence encoding a protein normally present in human milk,
- (b) cultivating the plant under seed-maturation conditions, and
- (c) harvesting the seeds from the plant, wherein the milk protein constitutes at least 3% of the total soluble protein contained in the harvested seeds.
A method of producing a recombinant milk protein in monocot plant seeds comprising the steps of:
- (a) obtaining a monocot plant stably transformed with a chimeric gene having (i) a seed maturation-specific promoter, (ii) operably linked to said promoter, a leader DNA sequence encoding a monocot seed-specific transit sequence capable of targeting a linked polypeptide to an endosperm-cell organelle, and (iii) the protein-coding sequence comprising of SEQ ID NO:1,
- (b) cultivating the plant under seed-maturation conditions, and
- (c) harvesting the seeds from the plant, wherein the milk protein constitutes at least 3% of the total soluble protein contained in the harvested seeds.
Dependencies are inferred from the claim text.