Use of particulate labels in bioanalyte detection methods
AssigneeTrellis Bioscience, LLC
InventorsLawrence M. Kauvar, Remy Cromer, William D. Harriman, Ellen J. Collarini
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Abstract
New applications for the use of distinguishable particulate labels available in a variety of hues and sized in the submicron range are described. These applications include profiling of cellular components, obtaining secretion patterns, identifying a multiplicity of components in chromatographic or electrophoretic techniques and identification of desired immunoglobulin secreting cells.
Claims18
A method to determine the presence, absence or amount of each of a multiplicity of components released from a single cell and/or the nature of at least one released component from the single cell, which method comprises permeabilizing or lysing the single cell or allowing the single cell to secrete cellular components,
- disposing the released components, on a planar sample surface, contacting the released components disposed on said surface with a multiplicity of particulate labels having diameters of 10-500 nm with different hues, wherein each differently hued particle is conjugated to specific binding partner for a cellular component or portion thereof;
- and
- wherein particulate labels bound to components are retained but particulate labels not bound to components are not retained;
- removing particulate labels not bound to components;
- and
- observing the number and nature of individual particulate labels that are bound to the released components on the sample surface by means of microscopic observation,
- whereby the presence, absence or amount of each of a multiplicity of released components and/or the nature of at least one released component from said single cell are determined.
Dependencies are inferred from the claim text.