# US 7,411,117 B2 · Inbred corn line BE9515

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- Number: US 7,411,117 B2
- Document ID: US7411117B2
- Status: Granted
- Kind: B2
- Application number: 11/195,324
- Filed: 2005-08-02
- Published: 2007-02-08
- Granted: 2008-08-12
- Earliest claimed: 2005-08-02
- Assignees: Dow AgroScience LLC
- Inventors: Kermit Berg Bohning
- Web page: https://patentgrep.com/patent/US7411117B2

## CPC

- A01H 6/4684: {Zea mays [maize]}
- A01H 5/10: Seeds
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

An inbred corn line, designated BE9515, the plants and seeds of the inbred corn line BE9515, methods for producing a corn plant, either inbred or hybrid, produced by crossing the inbred corn line BE9515 with itself or with another corn plant, and hybrid corn seeds and plants produced by crossing the inbred line BE9515 with another corn line or plant and to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic corn plants produced by that method. This invention also relates to inbred corn lines derived from inbred corn line BE9515, to methods for producing other inbred corn lines derived from inbred corn line BE9515 and to the inbred corn lines derived by the use of those methods.

## Claims

### Claim 1

A seed of corn inbred line designated BE9515, or a part thereof, representative seed of the line having been deposited under ATCC Accession No. PTA-6069.

### Claim 2 (depends on 1)

The seed or a part thereof of claim 1, wherein the part is selected from the group consisting of pericarp, germ and endosperm.

### Claim 3 (depends on 1)

The seed or a part thereof of claim 1, wherein the seed further comprises a coating.

### Claim 4 (depends on 1)

An essentially homogeneous population of seed consisting essentially of the corn seed of claim 1.

### Claim 5 (depends on 1)

A method for producing inbred corn seed, comprising:
(a) planting the seed of claim 1 in proximity to itself or to a seed from different line;
(b) growing plants from the seed under pollinating conditions; and,
(c) harvesting resultant seed.

### Claim 6 (depends on 1, 5)

The method of claim 5, further comprising pre-treating the seed of claim 1 with an agricultural chemical before performing step (a).

### Claim 7 (depends on 5)

The method of claim 5, further comprising treating the growing plants or soil surrounding the growing plants with an agricultural chemical.

### Claim 8 (depends on 1)

A corn plant produced by growing the seed of claim 1.

### Claim 9 (depends on 8)

A part of the corn plant of claim 8, selected from the group consisting of a plant cell, a plant protoplast, a plant callus, a plant clump, an embryo, a pollen, an ovule, a flower, a kernel, a seed, an ear, a cob, a leaf, a husk, a stalk, a root, a root tip, a brace root, a lateral tassel branch, an anther, a tassel, a glume, a tiller and a silk.

### Claim 10 (depends on 8)

Pollen of the plant of claim 8.

### Claim 11 (depends on 8)

An ovule of the plant of claim 8.

### Claim 12 (depends on 8)

A corn plant having all of the physiological and morphological characteristics of the corn plant of claim 8.

### Claim 13 (depends on 8)

An essentially homogenous population of corn plants consisting essentially the corn plant of claim 8.

### Claim 14 (depends on 13)

The essentially homogenous population of corn plants of claim 13, wherein the population is present in a field.

### Claim 15

A method for producing a progeny corn seed, comprising:
(a) crossing a plant of inbred corn line BE9515, representative seed of the line having been deposited under ATCC Accession No. PTA-6069, with another different corn plant to yield progeny corn seed.

### Claim 16 (depends on 15)

The method of claim 15, wherein the other, different corn plant is an inbred corn plant.

### Claim 17 (depends on 15)

The method of claim 15, further comprising:
(b) growing the progeny corn seed to produce progeny plant; and
(c) growing the progeny plant under self-pollinating or sib-pollinating conditions from 5 to 7 generations; and
(d) harvesting resultant seed.

### Claim 18 (depends on 15)

The method of claim 15, further comprising selecting plants obtained from growing at least one generation of the progeny corn seed for a desirable trait.

### Claim 19

A method of introducing a desired trait into corn inbred line BE9515 comprising:
a) crossing BE9515 plants grown from seed deposited under ATCC Accession No. 6069, with plants of another corn line that comprise a desired trait to produce F1 progeny plants;
(b) selecting F1 progeny plants that have the desired trait;
(c) crossing selected progeny plants with BE9515 plants to produce backcross progeny plants;
(d) selecting for backcross progeny plants that comprise the desired trait and physiological and morphological characteristics of corn inbred line BE9515; and
(e) performing steps (c) and (d) three or more times in succession to produce a selected fourth or higher backcross progeny plants that comprise the desired trait and all of the physiological and morphological characteristics of corn inbred line BE9515 listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.

### Claim 20 (depends on 19)

The method of claim 19, wherein the plants of the other corn line comprise a desired trait selected from the group consisting of male sterility, herbicide resistance, insect resistance, and resistance to bacterial, fungal and viral disease.

### Claim 21 (depends on 8)

A method for producing a corn plant, comprising:
a) crossing the inbred corn plant of claim 8 with another different corn plant.

### Claim 22 (depends on 21)

The method of claim 21, wherein the different corn plant is a haploid inducer.

### Claim 23 (depends on 8)

A method for producing a corn plant, comprising:
a) inducing a mutation in the corn plant, or a part thereof, of claim 8.

### Claim 24 (depends on 23)

The method of claim 23, wherein the mutation is artificially induced by a method selected from the group consisting of elevated temperature, long-term seed storage, tissue culture conditions, radiation, and chemical mutagenesis.

### Claim 25

A method for producing an inbred corn plant, comprising:
a) growing first generation hybrid corn plants having BE9515 as a parent corn plant;
b) selfing the first generation hybrid corn plants or crossing the first generation hybrid corn plants with different corn plants to yield progeny corn seed;
c) growing the progeny corn seed of step b) to yield further progeny corn seed; and
d) repeating steps b) and c) from 0 to 7 times to generate an inbred corn plant.

### Claim 26

A method for producing inbred BE9515, representative seed of the line having been deposited under ATCC Accession No. PTA-6069, comprising:
(a) planting a collection of seed comprising seed of a hybrid, one of whose parents is a inbred BE9515, the collection also comprising seed of the inbred BE9515;
(b) growing plants from the collection of seed;
(c) identifying inbred BE9515 parent plants by their decreased vigor relative to the hybrid plants produced from the hybrid seed;
(d) controlling pollination in a manner which preserves the homozygosity of the inbred parent plant; and
(e) harvesting the resultant seed of inbred BE9515.

### Claim 27 (depends on 26)

A method for producing an inbred plant comprising: sib-pollinating plants obtained by growing the harvested resultant seed of step (e) of claim 26.

### Claim 28 (depends on 8)

A method for producing a hybrid corn seed comprising crossing a first inbred parent corn plant with a second inbred parent corn plant and harvesting resultant hybrid corn seed, wherein the first inbred corn plant or the second inbred corn plant is the corn plant of claim 8.

### Claim 29 (depends on 8)

A method for producing a hybrid corn seed comprising the steps of:
(a) planting in pollinating proximity seeds of the first and the second inbred corn plants, wherein the first inbred corn plant or the second inbred corn plant is the corn plant of claim 8;
(b) cultivating the seeds of the first and the second inbred corn plants into plants that bear flowers;
(c) preventing self pollination of the first or the second inbred corn plant;
(d) allowing cross-pollination to occur between the first and second inbred corn plants; and
(e) harvesting seeds resulting from said cross-pollination.

### Claim 30 (depends on 29)

The method according to claim 29, wherein the first inbred corn plant or the second inbred parent female parent.

### Claim 31 (depends on 29)

The method according to claim 29, wherein the first inbred corn plant or the second inbred parent male parent.

### Claim 32 (depends on 29)

An F1 hybrid corn seed produced by the method of claim 29.

### Claim 33 (depends on 32)

A hybrid corn plant, or a part thereof, producing by growing said hybrid corn seed of claim 32.

### Claim 34 (depends on 9)

A method for producing a hybrid corn seed comprising crossing a first inbred parent corn plant with a second inbred parent corn plant and harvesting the resultant hybrid corn seed, wherein the first inbred corn plant or the second inbred corn plant is a progeny plant of a cross of the corn plant of claim 9 and another inbred corn plant.

### Claim 35 (depends on 8)

An F1 hybrid seed produced by crossing the inbred corn plant according to claim 8 with another, different corn plant.

### Claim 36 (depends on 35)

A hybrid corn plant, or a part thereof, produced by growing the hybrid corn seed of claim 35.

### Claim 37

A method for producing a BE9515-derived corn plant, comprising:
a) crossing inbred corn line BE9515, representative seed of said line having been deposited under ATCC Accession No. 6069, with a second corn plant to yield progeny corn seed; and
b) growing said progeny corn seed, under plant growth conditions, to yield said BE9515-derived corn plant.

### Claim 38 (depends on 37)

The method of claim 37, further comprising:
c) crossing the BE9515-derived corn plant with itself or with another corn plant to yield additional BE9515-derived progeny corn seed;
d) growing said progeny corn seed of step c) under plant growth conditions, to yield additional BE9515-derived corn plants; and
e) repeating the crossing and growing steps of c) and d) from 0 to 7 times to generate further BE9515-derived corn plants.

### Claim 39 (depends on 37)

The method of claim 37, further comprising utilizing plant tissue culture methods and/or haploid breeding to derive progeny of said BE9515-derived corn plant.

### Claim 40 (depends on 8)

A tissue culture of regenerable cells produced from the corn plant of claim 8.

### Claim 41 (depends on 40)

The tissue culture according to claim 40, wherein the tissue culture is produced from cells or protoplasts from a tissue selected from the group consisting of leaves, pollen, embryos, roots, root tips, anthers, silks, flowers, kernels, ears, cobs, husks and stalks.

### Claim 42 (depends on 40)

A corn plant regenerated from the tissue culture of claim 40, wherein the regenerated plant has of expressing all the morphological and physiological characteristics of inbred line BE9515.

### Claim 43 (depends on 8)

A corn plant with all of the physiological and morphological characteristics of corn inbred BE9515, wherein the corn plant is produced by a tissue culture process using the corn plant of claim 8 as the starting material for such a process.

### Claim 44 (depends on 33)

A tissue culture of regenerable cells from the hybrid corn plant of claim 33.

### Claim 45

A method for regenerating a corn plant comprising the steps of:
(a) culturing an explant comprising a tissue selected from the group consisting of a tissue obtained from inbred corn plant BE9515 representative seed having been deposited under ATCC Accession No. PTA-6069, an immature tissue obtained from a hybrid corn plant having BE9515 as a parent, and a BE9515-derived corn plant; and,
(b) initiating regeneration.

### Claim 46 (depends on 45)

The process of claim 45, wherein the explant is an immature tissue.

### Claim 47 (depends on 8)

The corn plant of claim 8, wherein the BE9515 plant is male sterile.

### Claim 48 (depends on 8)

The corn plant of claim 8, wherein the male sterile BE9515 plant is a cytoplasmic male sterile plant.

### Claim 49 (depends on 8)

A method for producing a male sterile BE9515 corn plant, comprising:
(a) crossing the inbred corn plant of claim 8, with a cytoplasmic male sterile corn plant to produce a male sterile BE9515 corn plant.

### Claim 50 (depends on 8)

The corn plant, or a part thereof, of claim 8, wherein the plant or part thereof has been transformed so that its genetic material contains one or more transgenes operably linked to one or more regulatory elements.

### Claim 51 (depends on 8)

A method for producing a corn plant that contains in its genetic material one or more transgenes, comprising crossing the corn plant of claim 8 with either a second plant of another corn line, or a transformed corn plant of the line BE9515, each containing said one or more transgenes, so that the progeny that result from the cross contains the one or more transgenes operably linked to a regulatory element.

### Claim 52 (depends on 8)

The corn plant, or a part thereof, according to claim 8, further comprising one or more transgenes.

### Claim 53 (depends on 8)

The corn plant, or a part thereof, according to claim 8, wherein the plant or a part thereof has been transformed so that its genetic material contains one or more transgenes operably linked to one or more regulatory elements.

### Claim 54 (depends on 53)

The corn plant according to claim 53, wherein said one or more transgenes comprises a gene conferring upon said maize plant tolerance to a herbicide.

### Claim 55 (depends on 54)

The corn plant according to claim 54, wherein said herbicide is glyphosate, gluphosinate, a sulfonylurea or an imidazolinone herbicide, a hydroxyphenylpyruvate dioxygenase inhibitor or a protoporphyrinogen oxidase inhibitor.

### Claim 56 (depends on 52)

The corn plant according to claim 52, wherein the one or more transgenes comprises a gene conferring upon said maize plant insect resistance, disease resistance or virus resistance.

### Claim 57 (depends on 56)

The corn plant according to claim 56, wherein the gene conferring upon the corn plant insect resistance is a Bacillus thuringiensis gene.

### Claim 58 (depends on 52)

Seed of the plant according to claim 52.

### Claim 59 (depends on 51)

A corn plant, or a part thereof, produced by the method of claim 51.

### Claim 60 (depends on 8)

An inbred corn plant, or a part thereof, comprising an SSR genetic marker profile; wherein at least 90% of the genetic markers are in accordance with the profile shown in Table 5; and wherein the plant has all of the morphological and physiological characteristics of the corn plant or a part thereof of claim 8.

### Claim 61

A method for developing a corn plant in a corn plant breeding program comprising, utilizing the inbred corn plant BE9515 genetic marker dataset shown in Table 5.

### Claim 62 (depends on 61)

The method of claim 61, wherein the step (a) comprises comparing the inbred corn plant genetic marker dataset to a hybrid corn plant genetic marker dataset to determine a relationship between the genetic marker datasets.