# US 7,411,115 B2 · Sweet potato sporamin gene promoter

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- Number: US 7,411,115 B2
- Document ID: US7411115B2
- Status: Granted
- Kind: B2
- Application number: 10/989,719
- Filed: 2004-11-15
- Published: 2005-09-08
- Granted: 2008-08-12
- Earliest claimed: 2002-03-13
- Assignees: Academia Sinica
- Inventors: Su-May Yu
- Web page: https://patentgrep.com/patent/US7411115B2

## CPC

- C12N 15/8257: {for the production of primary gene products, e.g. pharmaceutical products, interferon}
- C12N 15/8223: {Vegetative tissue-specific promoters}
- C12N 15/8225: {Leaf-specific, e.g. including petioles, stomata}
- C12N 15/8226: {Stem-specific, e.g. including tubers, beets}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A transformed tuberous plant cell or a transgenic tuberous plant containing a heterologous nucleic acid that includes a sweet potato sporamin promoter operably linked to a sequence encoding a polypeptide.

## Claims

### Claim 1

A transformed tuberous plant cell comprising a heterologous nucleic acid that contains a sweet potato sporamin promoter operably linked to a sequence encoding a polypeptide, wherein the sweet potato sporamin promoter includes SEQ ID NO:1.

### Claim 2 (depends on 1)

The transformed tuberous plant cell of claim 1, wherein the polypeptide is an Escherichia coli phytase.

### Claim 3 (depends on 1)

The transformed tuberous plant cell of claim 1, wherein the sequence encoding the polypeptide is linked to an upstream sequence encoding a propeptide, and the sequence encoding the propeptide is linked to a further upstream sequence encoding a signal peptide, wherein both the propeptide and the signal peptide are from a sweet potato sporamin protein.

### Claim 4 (depends on 3)

The transformed tuberous plant cell of claim 3, wherein the signal peptide is SEQ ID NO:3 and the propeptide is SEQ ID NO:4.

### Claim 5 (depends on 1)

The transformed tuberous plant cell of claim 1, wherein the tuberous plant is potato.

### Claim 6 (depends on 5)

The transformed tuberous plant cell of claim 5, wherein the polypeptide is an Escherichia coli phytase.

### Claim 7 (depends on 5)

The transformed tuberous plant cell of claim 5, wherein the sequence encoding the polypeptide is linked to an upstream sequence encoding a propeptide, and the sequence encoding the propeptide is linked to a further upstream sequence encoding a signal peptide, wherein both the propeptide and the signal peptide are from a sweet potato sporamin protein.

### Claim 8 (depends on 7)

The transformed tuberous plant cell of claim 7, wherein the signal peptide is SEQ ID NO:3 and the propeptide is SEQ ID NO:4.

### Claim 9

A transgenic tuberous plant whose genome comprises a heterologous nucleic acid that contains a sweet potato sporamin promoter operably linked to a sequence encoding a polypeptide, wherein the sweet potato sporamin promoter includes SEQ ID NO:1.

### Claim 10 (depends on 9)

The transgenic tuberous plant of claim 9, wherein the polypeptide is an Escherichia coli phytase.

### Claim 11 (depends on 9)

The transgenic tuberous plant of claim 9, wherein the sequence encoding the polypeptide is linked to an upstream sequence encoding a propeptide, and the sequence encoding the propeptide is linked to a further upstream sequence encoding a signal peptide, wherein both the propeptide and the signal peptide are from a sweet potato sporamin protein.

### Claim 12 (depends on 11)

The transgenic tuberous plant of claim 11, wherein the signal peptide is SEQ ID NO:3 and the propeptide is SEQ ID NO:4.

### Claim 13 (depends on 9)

The transgenic tuberous plant of claim 9, wherein the polypeptide is expressed in the leaf, stem, and microtuber of the transgenic tuberous plant cultured in medium.

### Claim 14 (depends on 9)

The transgenic tuberous plant of claim 9, wherein the polypeptide is expressed in the leaf, petiole, and tuber of the transgenic tuberous plant grown in soil.

### Claim 15 (depends on 9)

The transgenic tuberous plant of claim 9, wherein the tuberous plant is potato.

### Claim 16 (depends on 15)

The transgenic tuberous plant of claim 15, wherein the polypeptide is an Escherichia coli phytase.

### Claim 17 (depends on 15)

The transgenic tuberous plant of claim 15, wherein the sequence encoding the polypeptide is linked to an upstream sequence encoding a propeptide, and the sequence encoding the propeptide is linked to a further upstream sequence encoding a signal peptide, wherein both the propeptide and the signal peptide are from a sweet potato sporamin protein.

### Claim 18 (depends on 17)

The transgenic tuberous plant of claim 17, wherein the signal peptide is SEQ ID NO:3 and the propeptide is SEQ ID NO:4.

### Claim 19 (depends on 15)

The transgenic tuberous plant of claim 15, wherein the polypeptide is expressed in the leaf, stem, and microtuber of the transgenic tuberous plant cultured in medium.

### Claim 20 (depends on 15)

The transgenic tuberous plant of claim 15, wherein the polypeptide is expressed in the leaf, petiole, and tuber of the transgenic tuberous plant grown in soil.