# US 7,411,112 B2 · Maize promoter named CRWAQ81

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- Number: US 7,411,112 B2
- Document ID: US7411112B2
- Status: Granted
- Kind: B2
- Application number: 10/961,629
- Filed: 2004-10-08
- Published: 2005-05-05
- Granted: 2008-08-12
- Earliest claimed: 2003-10-09
- Assignees: Pioneer Hi-Bred International, Inc.; E.I. du Pont de Nemours and Company; New York University
- Inventors: Scott Diehn; Albert Laurence Lu; Steven W. Ritchie; Lynne Sims; Kim R. Ward; Philip Benfey; Jee W. Jung
- Web page: https://patentgrep.com/patent/US7411112B2

## CPC

- C12N 15/8227: {Root-specific}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding CRWAQ81. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

## Claims

### Claim 1

An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2;
b) a nucleotide sequence comprising a plant promoter sequence that comprises at least 800 contiguous nucleotides of the plasmids deposited as Patent Deposit No. PTA-5126; and
c) a nucleotide sequence comprising at least 800 contiguous nucleotides of the sequence set forth in SEQ ID NO:1 or SEQ ID N0:2, wherein said sequence initiates transcription in a plant cell.

### Claim 2 (depends on 1)

An expression cassette comprising a nucleotide sequence of claim 1 operably linked to a heterologous nucleotide sequence of interest.

### Claim 3 (depends on 2)

A vector comprising the expression cassette of claim 2.

### Claim 4 (depends on 2)

A plant cell having stably incorporated into its genome the expression cassette of claim 2.

### Claim 5 (depends on 4)

The plant cell of claim 4, wherein said plant cell is from a monocot.

### Claim 6 (depends on 5)

The plant cell of claim 5, wherein said monocot is maize.

### Claim 7 (depends on 4)

The plant cell of claim 4, wherein said plant cell is from a dicot.

### Claim 8 (depends on 2)

A plant having stably incorporated into its genome the expression cassette of claim 2.

### Claim 9 (depends on 8)

The plant of claim 8, wherein said plant is a monocot.

### Claim 10 (depends on 9)

The plant of claim 9, wherein said monocot is maize.

### Claim 11 (depends on 8)

The plant of claim 8, wherein said plant is a dicot.

### Claim 12 (depends on 8)

A transgenic seed of the plant of claim 8.

### Claim 13 (depends on 8)

The plant of claim 8, wherein the heterologous nucleotide sequence of interest encodes a gene product that confers herbicide, salt, pathogen, drought, cold, or insect resistance.

### Claim 14 (depends on 1)

An expression cassette comprising, in sequence, the nucleic acid molecule of claim 1 operably linked to a heterologous nucleotide sequence of interest, which is operably linked to a 3′ non-translated region including a polyadenylation signal.

### Claim 15 (depends on 14)

A vector comprising the expression cassette of claim 14.

### Claim 16 (depends on 14)

A plant cell having stably incorporated into its genome the expression cassette of claim 14.

### Claim 17 (depends on 14)

A plant having stably incorporated into its genome the expression cassette of claim 14.

### Claim 18 (depends on 17)

A transgenic seed of the plant of claim 17.

### Claim 19 (depends on 17)

The plant of claim 17, wherein the heterologous nucleotide sequence of interest encodes a gene product that confers herbicide, salt, pathogen, drought, cold, or insect resistance.

### Claim 20

A method for expressing a nucleotide sequence in a plant, said method comprising introducing into a plant cell an expression cassette, said expression cassette comprising a promoter and operably linked to said promoter a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2;
b) a nucleotide sequence comprising a plant promoter sequence that comprises at least 800 contiguous nucleotides of the plasmids deposited as Patent Deposit No. PTA-5126; and
c) a nucleotide sequence comprising at least 800 contiguous nucleotides of the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2, wherein said sequence initiates transcription in a plant cell.

### Claim 21 (depends on 20)

The method of claim 20, wherein said heterologous nucleotide sequence of interest is selectively expressed in roots.

### Claim 22 (depends on 20)

The method of claim 20, wherein said plant is a dicot.

### Claim 23 (depends on 20)

The method of claim 20, wherein said plant is a monocot.

### Claim 24 (depends on 20)

The method of claim 20, wherein said monocot is maize.

### Claim 25 (depends on 20)

The method of claim 20, wherein the heterologous nucleotide sequence of interest encodes a gene product that confers herbicide, salt, pathogen, drought, cold, or insect resistance.

### Claim 26

A method for expressing a nucleotide sequence in a plant cell, said method comprising introducing into said plant cell an expression cassette comprising a promoter operably linked to a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2;
b) a nucleotide sequence comprising a plant promoter sequence that comprises at least 800 contiguous nucleotides of the plasmids deposited as Patent Deposit No. PTA-5126; and
c) a nucleotide sequence comprising at least 800 contiguous nucleotides of the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2, wherein said sequence initiates transcription in a plant cell.

### Claim 27 (depends on 26)

The method of claim 26, wherein said plant cell is from a monocot.

### Claim 28 (depends on 27)

The method of claim 27, wherein said monocot is maize.

### Claim 29 (depends on 26)

The method of claim 26, wherein said plant cell is from a dicot.

### Claim 30 (depends on 26)

The method of claim 26, wherein the heterologous nucleotide sequence of interest encodes a gene product that confers herbicide, salt, pathogen, drought, cold, or insect resistance.

### Claim 31

A method for selectively expressing a nucleotide sequence in a plant root, said method comprising introducing into a plant cell an expression cassette, and regenerating a transformed plant from said plant cell, said expression cassette comprising a promoter and a heterologous nucleotide sequence operably linked to said promoter, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2;
b) a nucleotide sequence comprising a plant promoter sequence that comprises at least 800 contiguous nucleotides of the plasmids deposited as Patent Deposit No. PTA-5126; and
c) a nucleotide sequence comprising at least 800 contiguous nucleotides of the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2, wherein said sequence initiates transcription in a plant cell.

### Claim 32 (depends on 31)

The method of claim 31, wherein expression of said heterologous nucleotide sequence of interest alters the phenotype of said plant.

### Claim 33 (depends on 31)

The method of claim 31, wherein said plant is a monocot.

### Claim 34 (depends on 33)

The method of claim 33, wherein said monocot is maize.

### Claim 35 (depends on 31)

The method of claim 31, wherein said plant is a dicot.

### Claim 36 (depends on 31)

The method of claim 31, wherein the heterologous nucleotide sequence of interest encodes a gene product that confers herbicide, salt, pathogen, drought, cold, or insect resistance.