# US 7,411,059 B2 · Production of syringyl lignin in gymnosperms

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- Number: US 7,411,059 B2
- Document ID: US7411059B2
- Status: Granted
- Kind: B2
- Application number: 10/681,878
- Filed: 2003-10-09
- Published: 2005-04-07
- Granted: 2008-08-12
- Earliest claimed: 1996-12-16
- Assignees: Arborgen, LLC
- Inventors: Vincent L. Chiang; Daniel T. Carraway; Richard H. Smeltzer
- Web page: https://patentgrep.com/patent/US7411059B2

## CPC

- C12N 9/0077: {with a reduced iron-sulfur protein as one donor (1.14.15)}
- C12N 9/1007: {Methyltransferases (general) (2.1.1.)}
- C12N 9/93: {Ligases (6)}
- C12N 15/8222: {Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation}
- C12N 15/8223: {Vegetative tissue-specific promoters}
- C12N 15/8255: {involving lignin biosynthesis}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to a method for producing syringyl lignin in gymnosperms. The production of syringyl lignin in gymnosperms is accomplished by genetically transforming a gymnosperm genome, which does not normally contain genes which code for enzymes necessary for production of syringyl lignin, with DNA which codes for enzymes found in angiosperms associated with production of syringyl lignin. The expression of the inserted DNA is mediated using host promoter regions in the gymnosperm. In addition, genetic sequences which code for gymnosperm lignin anti-sense mRNA may be incorporated into the gymnosperm genome in order to suppress the formation of the less preferred forms of lignin in the gymnosperm such as guaiacyl lignin.

## Claims

### Claim 1

An isolated promoter comprising SEQ ID NO: 10.
