# US 7,410,946 B2 · Method for treating damage in intact skin comprising administering a composition comprising basic milk growth factors in amounts higher than those in milk

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- Number: US 7,410,946 B2
- Document ID: US7410946B2
- Status: Granted
- Kind: B2
- Application number: 10/332,705
- Filed: 2001-07-13
- Published: 2004-04-29
- Granted: 2008-08-12
- Earliest claimed: 2000-07-13
- Assignees: Novozymes Biopharma Au Limited
- Inventors: Timothy Edward Rayner; Allison June Cowin; David Andrew Belford
- Web page: https://patentgrep.com/patent/US7410946B2

## CPC

- A61K 8/986: {Milk; Derivatives thereof, e.g. butter}
- A61K 35/20: Milk; Whey; Colostrum
- A61K 38/1875: {Bone morphogenic factor; Osteogenins; Osteogenic factor; Bone-inducing factor}
- A61P 3/00: Drugs for disorders of the metabolism (of the blood or the extracellular fluid A61P7/00)
- A61P 3/10: for hyperglycaemia, e.g. antidiabetics
- A61P 13/12: of the kidneys
- A61P 15/12: for climacteric disorders
- A61P 17/00: Drugs for dermatological disorders
- A61P 17/02: for treating wounds, ulcers, burns, scars, keloids, or the like
- A61P 17/14: for baldness or alopecia
- A61P 17/16: Emollients or protectives, e.g. against radiation
- A61P 31/18: for HIV
- A61P 35/00: Antineoplastic agents
- A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- A61Q 19/00: Preparations for care of the skin
- A61Q 19/02: for chemically bleaching or whitening the skin
- A61Q 19/08: Anti-ageing preparations
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS

## Abstract

This invention relates to a method for treating skin damage, comprising the step of administration of a pharmaceutical or dermatological composition of basic milk factors to the area of the skin of a subject in need of such treatment. The invention further relates to a method for cosmetically treating the aged appearance of skin. The skin damage may be the result of normal biological ageing, environmental factors, dermatological disorders, medical treatments, surgical treatments and/or medical conditions. The aged appearance of skin may be attributed to wrinkles, blemishes, sagging, hyperpigmentation, changes to skin thickness and/or a rough appearance. In a preferred embodiment, the subject in need of such treatment requires the enhancement of the youthful appearance of their skin. The invention further relates to compositions for the treated of these conditions.

## Claims

### Claim 1

A method for treating skin damage, the method comprising: topically administering to an area of substantially intact skin of a mammal in need thereof an effective amount of a composition for increasing dermal cellularity and/or stimulating collagen production, and/or inhibiting matrix-metalloproteinase 1 production in the skin, the composition comprising a plurality of basic milk factors having basic to approximately neutral isoelectric points, the milk factors being present at concentrations greater than that found in the lactational secretion from which they were derived and separated from the lactational secretion from which they were derived, the plurality of basic milk factors comprising at least one basic milk factor that is insulin-like growth factor 1 (IGF-1) or transforming growth factor β2 (TGF-β2), the basic milk factors having a molecular weight of at least approximately 5,000 Da, wherein upon administration of the composition the at least one basic milk factor having a molecular weight of at least approximately 5000 Da passes through the uppermost layers of skin having barrier function to exert a biological effect on an underlying competent cell.

### Claim 2 (depends on 1)

The method according to claim 1 wherein the total concentration of basic milk factors is greater than or equal to about 0.25 mg/mL of composition.

### Claim 3 (depends on 1)

The method according to claim 1 wherein the total concentration of basic milk factors is greater than or equal to about 0.5 mg/mL of composition.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the composition is obtained by a method comprising adsorbing the milk factors to a cation exchange matrix, eluting the milk factors from the matrix, acidifying and concentrating the eluate.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the method stimulates production of collagen in the substantially intact skin.

### Claim 6 (depends on 1)

The method according to claim 1, wherein the method increases dermal cellularity in the substantially intact skin.

### Claim 7 (depends on 1)

The method according to claim 1, wherein the method inhibits production of matrix metalloproteinase 1 in the substantially intact skin.