Method and assay for detection of residues
AssigneeCharm Sciences, Inc.
InventorsSteven J. Saul, Robert J. Markovsky, David W. Douglas
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Abstract
Embodiments described herein include methods and assays for detecting an analyte in a sample using a plurality of control zone capture agents. Some embodiments include detection of multiple analytes in a sample utilizing a plurality of analyte binders and a control zone containing multiple control zone capture agents. In some embodiments, the multiple control zone capture agents capture a plurality of binders within one control zone. Test results are determined by comparison of the control zone signal to a test zone signal.
Claims21
A lateral flow device for detecting a plurality of analytes in a test solution, the test solution including a sample and more than one binder, the binder configured for generating a detectable signal and capable of combining with an analyte present in the sample to form a binder-analyte complex, the device comprising:
- a test strip configured to allow the test solution to flow, the test strip comprising:
- (i) more than one test zone, each test zone having immobilized thereon a test zone capture agent that can capture at least one of the binders that has not formed a binder-analyte complex;
- and
- (ii) a single control zone having a plurality of control zone capture agents combined together within the control zone, each of the plurality of control zone capture agents having different binding affinities for the binders and each able to capture at least one of the binders, whether or not the binder has formed a binder-analyte complex,
- wherein capture of any of the binders at any of the test zones and the control zone results in a detectable signal and wherein a greater signal in the control zone as compared to any one of the test zones indicates a positive result.
Dependencies are inferred from the claim text.