# US 7,410,783 B2 · Method for producing biodegradable polyester

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- Number: US 7,410,783 B2
- Document ID: US7410783B2
- Status: Granted
- Kind: B2
- Application number: 11/432,354
- Filed: 2006-05-12
- Published: 2007-11-15
- Granted: 2008-08-12
- Earliest claimed: 2006-05-12
- Assignees: Tatung University
- Inventors: C. Will Chen; Ting-Yen Huang; Trong-Ming Don
- Web page: https://patentgrep.com/patent/US7410783B2

## CPC

- C12P 7/625: Polyesters of hydroxy carboxylic acids
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}

## Abstract

The present invention relates to a method to produce biodegradable polyester, which comprises the following steps: (a) mixing a carbon source, a nitrogen source and water into a reactive material to perform pretreatment; (b) providing a halophilic bacterium, a starting broth and culture with the reactive material; (c) feeding the reactive material; and (d) extracting the polyester from the fermentation broth; wherein, the bacterium has a salt tolerance of 10-30%.

## Claims

### Claim 1

A method for producing biodegradable polyester, comprising steps of:
(a) mixing a carbon source, a nitrogen source and water into a reactive mixture to perform pretreatment;
(b) mixing a halophilic bacterium, a starting broth, and the reactive mixture together to form a reactive material, and culture the reactive material for fermentation;
(c) feeding the reactive material; and
(d) extracting the polyester from the fermented broth;
wherein the bacterium has salt tolerance of 10-30%.

### Claim 2 (depends on 1)

The method of claim 1, wherein the carbon source of step (a) is at least one selected from a group consisting of: cassava starch, cornstarch, potato starch, grass family starch, pulse family starch, wheat starch, rice bran, wheat bran, glucose, and molasses.

### Claim 3 (depends on 1)

The method of claim 1, wherein the nitrogen source of step (a) is at least one selected from a group consisting of: yeast extracts, soy bean extracts, ammonium sulfate, and whey.

### Claim 4 (depends on 1)

The method of claim 1, wherein the pretreatment in step (a) refers to the degradation reaction of contents of the mixture.

### Claim 5 (depends on 4)

The method of claim 4, wherein the degradation reaction refers to treatment by amylase and an extruder.

### Claim 6 (depends on 5)

The method of claim 5, wherein the amylase is selected from a group consisting of: plant α-amylase, microbial α-amylase, β-amylase, isoamylase, glucoamylase, pullulanase, cyclodextrin glucanotransferase, β-fructofuranosidase, and glucoisomerase.

### Claim 7 (depends on 6)

The method of claim 6, wherein the temperature range in which the amylases function is 25-110° C.

### Claim 8 (depends on 5)

The method of claim 5, wherein the extruder is a single-screw extruder.

### Claim 9 (depends on 1)

The method of claim 1, wherein the halobacteria in step (b) is Haloferax, Halobacterium, Haloarcula or Halococcus.

### Claim 10 (depends on 9)

The method of claim 9, wherein the halobacteria in step (b) is Haloferax mediterranei.

### Claim 11 (depends on 1)

The method of claim 1, wherein conductivity in step (b) is controlled in order to adjust salt concentration in the reaction liquid.

### Claim 12 (depends on 11)

The method of claim 11, wherein the range of conductivity is 2-4 S/m.

### Claim 13 (depends on 1)

The method of claim 1, when pH value is between 6.5-7.5, step (b) is performed.

### Claim 14 (depends on 1)

The method of claim 1, wherein the fed reactive materials comprise a carbon source, a nitrogen source, and salts.