# US 7,410,770 B2 · Method for monitoring collagen type II degradation in cartilage

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- Number: US 7,410,770 B2
- Document ID: US7410770B2
- Status: Granted
- Kind: B2
- Application number: 10/507,498
- Filed: 2003-03-12
- Published: 2005-08-04
- Granted: 2008-08-12
- Earliest claimed: 2002-03-13
- Assignees: Universite De Liege
- Inventors: Jean-Yves Reginster; Michelle Deberg; Yves Henrotin; Stephan Christgau
- Web page: https://patentgrep.com/patent/US7410770B2

## CPC

- C07K 16/18: against material from animals or humans
- G01N 33/6887: {from muscle, cartilage or connective tissue}
- G01N 2333/78: Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
- Y10S 530/84: Bones; tendons; teeth; cartilage
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

A method for improving the diagnostic assessment of cartilage degenerative processes, and to provide means of monitoring the effects of therapeutical measures taken towards arthritic diseases in most mammals utilizes an immunoassay to detect fragments of collagen type II resulting from collagenase activity comprising an antibody directed against an epitope comprised in the amino acid sequence HRGYPGLDG, located in the helical region of collagen type II.

## Claims

### Claim 1

A method of qualitative or quantitative assay of unwound collagen type II fragments containing amino acid sequence HRGYPGLDG (SEQ ID NO: 1) in a biological sample comprising
contacting the fragments with an antibody that binds with an epitope in the amino acid sequence HRGYPGLDG (SEQ ID NO: 1) and
detecting resulting immunoreaction.

### Claim 2 (depends on 1)

The method of claim 1, wherein the epitope consists of five or more amino acids in the sequence.

### Claim 3 (depends on 1)

The method of claim 1, wherein the antibody does not react with wound collagen type II fragments.

### Claim 4 (depends on 1)

The method of claim 1, wherein immunoreaction is detected between the epitope and collagen type II fragments of less than 80 kDa.

### Claim 5 (depends on 4)

The method of claim 4, wherein the fragments are of less than 30 kDa.

### Claim 6 (depends on 4)

The method of claim 4, wherein the fragments are of less than 10 kDa.

### Claim 7 (depends on 1)

The method of claim 1, wherein the sample is a biological fluid.

### Claim 8 (depends on 7)

The method of claim 7, wherein the biological fluid is serum, urine, tear fluid, synovial fluid, subcutaneous interstitial fluid, a cell culture supernatant fluid, or a cell extract.

### Claim 9 (depends on 1)

The method of claim 1, wherein detected immunoreaction is indicative of degradation of cartilage.