# US 7,410,763 B2 · Multiplex data collection and analysis in bioanalyte detection > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 7,410,763 B2 - Document ID: US7410763B2 - Status: Granted - Kind: B2 - Application number: 11/216,112 - Filed: 2005-09-01 - Published: 2007-03-01 - Granted: 2008-08-12 - Earliest claimed: 2005-09-01 - Assignees: Intel Corporation - Inventors: Xing Su; Lei Sun; Mineo Yamakawa; Jingwu Zhang; Qing Ma; Tae-Woong Koo; Richard Jones - Web page: https://patentgrep.com/patent/US7410763B2 ## CPC - G01N 33/53: Immunoassay; Biospecific binding assay; Materials therefor - B82Y 15/00: Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors - B82Y 30/00: Nanotechnology for materials or surface science, e.g. nanocomposites - G01N 33/54366: {Apparatus specially adapted for solid-phase testing} - G01N 33/5438: {Electrodes} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES ## Abstract Method and device to collect multiplex data simultaneously in analyte detection and analyze the data by experimentally trained software (machine-learning) is disclosed. Various ways (magnetic particles and microcoils) are disclosed to collect multiple reporter (tag) signals. Multiplex detection can increase the biomolecule analysis efficiency by using small sample size and saving assay reagents and time. Machine learning and data analysis schemes are also disclosed. Multiple affinity binding partners, each labeled by a unique reporter, are contacted with a sample and a green spectrum is taken to detect multiple reporter signals. The spectrum is deconvoluted by experimentally trained software to identify multiple analytes. ## Claims ### Claim 1 A device for data collection comprising a beam emitter, a microfluidic channel (MFC), having an array of spots, a spectrometer, a detector; wherein the device has an ability to detect a binding complex comprising a first binding partner, a first reporter associated with the first binding partner, an analyte, a second binding partner, and a second reporter associated with the second binding partner, wherein the first reporter or the second reporter comprises a nanomaterial having more than 50% by weight of inorganic content, wherein the MFC comprises a microcoil or a Micro-Electro-Mechanical System (MEMS) device. ### Claim 2 A device for data collection comprising a beam emitter, a chamber to hold a microarray, a spectrometer and a detector: wherein the device has an ability to detect a binding complex comprising a first binding partner, a first reporter associated with the first binding partner, an analyte, a second binding partner, and a second reporter associated with the second binding partner, wherein the first reporter or the second reporter comprises a nanomaterial having more than 50% by weight of inorganic content, wherein the chamber comprises an optical switch to detect the beam transmitted through the microarray. ### Claim 3 (depends on 2) The device of claim 2, wherein the spectrometer is a waveguide based spectrometer to detect a phase shift of a Raman signal emitted by the sample. ### Claim 4 (depends on 3) The device of claim 3, wherein the detector is to detect at least a characteristic of the Raman signal emitted by the sample. ### Claim 5 (depends on 4) The device of claim 4, further comprising a microprocessor comprising software or a hardware to identify the Raman signal. ### Claim 6 (depends on 1) The device of claim 1, wherein the MFC comprises an inorganic support and an optically transparent cover. ### Claim 7 (depends on 1) The device of claim 1, further comprising an analyte attached to at least one of the immobilized binding partner and another binding partner attached to the analyte and a reporter. ### Claim 8 (depends on 1) The device of claim 1, wherein the beam emitter is to emit a beam comprising laser. ### Claim 9 (depends on 1) The device of claim 1, further comprising an optical waveguide between the beam emitter and the MFC. ### Claim 10 (depends on 1) The device of claim 1, wherein the MFC comprises a detection site to illuminate a sample comprising a reporter attached to an analyte. ### Claim 11 (depends on 1) The device of claim 1, wherein the spectrometer is a waveguide based spectrometer to create a phase shift in a Raman signal emitted by the sample. ### Claim 12 (depends on 1) The device of claim 1, wherein the detector is to detect at least a characteristic of a Raman signal emitted by the sample. ### Claim 13 (depends on 1) The device of claim 1, further comprising a microprocessor comprising software or a hardware to identify the Raman signal. ### Claim 14 (depends on 1) The device of claim 1, wherein the MFC comprises a plurality of first binding partners immobilized on spots in the MFC. ### Claim 15 (depends on 1) The device of claim 1, wherein the MFC further comprises a plurality of COINs or magnetic COINs immobilized on spots. ### Claim 16 (depends on 2) The device of claim 2, wherein the spectrometer is a waveguide based spectrometer to detect a phase shift of a Raman signal emitted by the sample. ### Claim 17 (depends on 2) The device of claim 2, wherein the detector is to detect at least a characteristic of a Raman signal emitted by the sample. ### Claim 18 (depends on 2) The device of claim 2, further comprising a microprocessor comprising software or a hardware to identify a Raman signal. ### Claim 19 (depends on 2) The device of claim 2, wherein the microarray comprises a plurality of first binding partners immobilized on spots on the microarray. ### Claim 20 (depends on 2) The device of claim 2, wherein the microarray further comprises a plurality of COINs immobilized on spots. ### Claim 21 (depends on 2) The device of claim 2, wherein the plurality of COINs comprise a magnetic COIN.