# US 7,410,638 B1 · Bacterial strains, genes and enzymes for control of bacterial diseases by quenching quorum-sensing signals > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 7,410,638 B1 - Document ID: US7410638B1 - Status: Granted - Kind: B1 - Application number: 10/362,569 - Filed: 2000-08-23 - Granted: 2008-08-12 - Earliest claimed: 2000-08-23 - Assignees: Institute of Molecular Agrobiology - Inventors: Lianhui Zhang; Yihu Dong; Haibao Zhang; Jinling Xu - Web page: https://patentgrep.com/patent/US7410638B1 ## CPC - C07K 14/195: from bacteria - A61P 31/04: Antibacterial agents - C12N 15/8281: {for bacterial resistance} - A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) ## Abstract The present invention relates to isolated nucleic acid molecules encoding an autoinducer inactivation protein, wherein the encoded protein comprises an amino acid sequence selected from the group consisting of 104HXHXDH109˜60aa˜H169˜21aa˜D191 and 103HXHXDH108˜72aa˜H180˜21aa˜D202, and to expression vectors and transformed plant and animal cells comprising the same. The proteins encoded by these nucleic acid molecules provide to a susceptible plant or animal increased resistance to a disease the virulence of which is regulated by autoinducers. Also provided are methods of increasing disease resistance in susceptible plants and animals. ## Claims ### Claim 1 An isolated nucleic acid molecule encoding a protein comprising 263 amino acids having the formula 102aa-amino acid motif-61aa, wherein the amino acid motif consists of HXHXDH-71aa-H-21aa-D, wherein said protein has autoinducer inactivation activity and wherein the protein has the amino acid sequence set forth in SEQ ID NO:11. ### Claim 2 (depends on 1) The isolated nucleic acid molecule of claim 1, wherein the nucleic acid molecule has the nucleotide sequence set forth in SEQ ID NO:1. ### Claim 3 (depends on 1) An expression vector comprising the nucleic acid molecule of claim 1. ### Claim 4 (depends on 2) An expression vector comprising the nucleic acid molecule of claim 2. ### Claim 5 An isolated protein comprising 263 amino acids having the formula 102aa-amino acid motif-61aa, wherein the amino acid motif consists of HXHXDH-71aa-H-21aa-D, wherein said protein has autoinducer inactivation activity and wherein the protein has the amino acid sequence set forth in SEQ ID NO:11. ### Claim 6 (depends on 5) A method of reducing bacterial damage to a plant or animal, which method comprises administering to a plant or animal in need of such reduction an effective amount of the protein of claim 5. ### Claim 7 (depends on 6) A method according to claim 6, wherein administration is to an animal. ### Claim 8 (depends on 7) A method according to claim 7, wherein the animal is a human. ### Claim 9 An isolated cell of a procaryote or eukaryote stably transformed with a nucleic acid molecule encoding a protein comprising 263 amino acids having the formula 102aa-amino acid motif-61aa, wherein the amino acid motif consists of HXHXDH-71aa-H-21aa-D, wherein said protein has autoinducer inactivation activity and wherein the protein has the amino acid sequence set forth in SEQ ID NO:11. ### Claim 10 (depends on 9) The isolated cell of claim 9, wherein the nucleic acid molecule has the nucleotide sequence set forth in SEQ ID NO:1.