Transgenic algae for delivering antigens to an animal
AssigneesPhycotransgenics L.L.C., The Ohio State University
InventorsRichard T. Sayre, Richard E. Wagner, Surasak Siripornadulsil, Carlos Farias
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Abstract
Delivery systems and methods are provided for delivering a biologically active protein to a host animal. The systems and methods provided include obtaining an algal cell transformed by an expression vector, the expression vector comprising a nucleotide sequence coding for the biologically active protein, operably linked to a promoter. In one illustrated embodiment, the biologically active protein is an antigenic epitope and upon administration to the animal the algal cell induces an immune response in the host animal.
Claims32
A delivery system for delivering a biologically active protein to a host animal comprising
- an algal cell transformed by an expression vector, the expression vector comprising
- a β-tubulin promoter of Chlamydomonas,
- a 3′-terminator from the Chlamydomonas low C2-induced membrane protein gene having the sequence of SEQ ID NO. 4, and
- a nucleotide sequence coding for the biologically active protein fused to an end of the low CO2-induced plasma membrane protein gene SEQ ID NO. 3, said fused nucleotide sequence positioned between the β-tubulin promoter and the 3′ terminator and operably linked to the β-tubulin promoter.
A method for delivering antigens to a host animal to induce an immune response in the host animal, the method comprising the steps of
- administering an immunogenic composition to the host animal wherein the composition comprises i) an algal cell transformed by an expression vector, and ii) the expression vector comprising a nucleotide sequence coding for an antigen comprising a holoprotein or protein fragment from a microorganism selected from the group consisting of a bacterium, a virus, a fungus, and a parasite, and
- inducing the immune response in the host animal.
Dependencies are inferred from the claim text.