# US 7,410,633 B2 · Measurement of protein synthesis rates in humans and experimental systems by use of isotopically labeled water

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- Number: US 7,410,633 B2
- Document ID: US7410633B2
- Status: Granted
- Kind: B2
- Application number: 11/233,549
- Filed: 2005-09-22
- Published: 2006-02-09
- Granted: 2008-08-12
- Earliest claimed: 2001-10-24
- Assignees: The Regents of the University of California
- Inventors: Marc K. Hellerstein
- Web page: https://patentgrep.com/patent/US7410633B2

## CPC

- G01N 33/68: involving proteins, peptides or amino acids {(involving lipoproteins G01N33/92)}
- G01N 33/6848: {Methods of protein analysis involving mass spectrometry}
- G01N 33/6893: {related to diseases not provided for elsewhere}
- G01N 33/92: involving lipids, e.g. cholesterol {, lipoproteins, or their receptors (steroid hormones G01N33/743)}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Provided herein are methods for measuring protein biosynthesis by using 2H2O or radioactive 3H2O and applicable uses thereof.

## Claims

### Claim 1

A method of determining the degradation rate of one or more proteins or peptides in an individual comprising the steps of:
(a) administering labeled water to an individual over a period of time sufficient for a label of said labeled water to be incorporated into said one or more proteins or peptides to form one or more labeled proteins or labeled peptides wherein said label is 2H or 18O;
(b) obtaining one or more bodily tissues or fluids from said individual, wherein said one or more bodily tissues or fluids comprise said one or more labeled proteins or labeled peptides; and
(c) detecting incorporation of said label in said one or more labeled proteins or labeled peptides to determine the degradation rate of said one or more proteins or peptides.

### Claim 2 (depends on 1)

The method of claim 1, wherein step (a) is discontinued prior to step (b).

### Claim 3 (depends on 1)

The method of claim 1, wherein said label is 2H.

### Claim 4 (depends on 1)

The method according to claim 1, comprising the additional step of partially purifying said one or more labeled proteins or labeled peptides from said one or more bodily tissues or fluids before step (c).

### Claim 5 (depends on 1)

The method according to claim 1, wherein said incorporation of said label is detected by mass spectrometry.

### Claim 6 (depends on 1)

The method according to claim 1, wherein said labeled water is administered orally.

### Claim 7 (depends on 1)

The method according to claim 1, wherein said individual is a human.

### Claim 8 (depends on 1)

The method according to claim 1, wherein said one or more proteins or peptides are selected from the group consisting of full length proteins or fragments of bone collagen, liver collagen, lung collagen, cardiac collagen, muscle myosin, serum hormone, plasma apolipoproteins, serum albumin, cloning factor, immunoglobulin, and mitochondrial protein.

### Claim 9

A method of determining the degradation rate of one or more proteins or peptides in an individual comprising:
(a) administering labeled water to an individual over a period of time sufficient for a label of said labeled water to be incorporated into said one or more proteins or peptides to form one or more labeled proteins or labeled peptides wherein said label is 2H or 18O;
(b) obtaining one or more bodily tissues or fluids from said individual, wherein said one or more bodily tissues or fluids comprise said one or more labeled proteins or labeled peptides;
(c) hydrolyzing said one or more labeled proteins or labeled peptides to produce one or more labeled amino acids or labeled oligopeptides; and
(d) detecting incorporation of said label in said one or more labeled amino acids or labeled oligopeptides to determine the degradation rate of said one or more proteins or peptides.

### Claim 10 (depends on 9)

The method of claim 9 wherein step (a) is discontinued prior to step (b).

### Claim 11 (depends on 9)

The method of claim 9 wherein said label is 2H.

### Claim 12 (depends on 9)

The method of claim 9, comprising the additional step of partially purifying said one or more labeled proteins or labeled peptides from said bodily tissues or fluids after step (b).

### Claim 13 (depends on 9)

The method according to claim 9, comprising the additional step of separating said one or more labeled amino acids or labeled oligopeptides by gas chromatography or HPLC after step (c).

### Claim 14 (depends on 9)

The method according to claim 9 wherein said incorporation of said label is detected by mass spectrometry.

### Claim 15 (depends on 9)

The method of claim 9 wherein said labeled water is administered orally.

### Claim 16 (depends on 9)

The method of claim 9 wherein said individual is a human.

### Claim 17 (depends on 9)

The method according to claim 9 wherein said one or more proteins or peptides are selected from the group consisting of full length proteins or fragments of bone collagen, liver collagen, lung collagen, cardiac collagen, muscle myosin, serum hormone, plasma apolipoproteins, serum albumin, cloning factor, immunoglobulin, and mitochondrial protein.