# US 7,410,560 B2 · Device for storage and delivery of standards for gel electrophoresis

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- Number: US 7,410,560 B2
- Document ID: US7410560B2
- Status: Granted
- Kind: B2
- Application number: 10/936,876
- Filed: 2004-09-08
- Published: 2005-04-21
- Granted: 2008-08-12
- Earliest claimed: 2003-09-11
- Assignees: Bio-Rad Laboratories, Inc.
- Inventors: Roger Provost; William Strong
- Web page: https://patentgrep.com/patent/US7410560B2

## CPC

- G01N 27/44717: {Arrangements for investigating the separated zones, e.g. localising zones}
- G01N 27/44704: {Details; Accessories}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Gel plugs that can be stored in a sealed condition and delivered individually to an electrophoresis gel as standards for electrophoretic separations are retained in a cartridge that is cleanly breakable into individual sections, each section retaining a gel plug of predefined dimensions with standard solute mixtures embedded in the plug. The separation of any single section from the cartridge exposes opposing edges of the gel plug which permits the user to insert an implement into the section interior and to urge the plug out of the section and into a well of an electrophoresis gel. All manipulations are performed without any need for contact between the user and the gel.

## Claims

### Claim 1

A gel plug cartridge for use as a source of standards in gel electrophoresis, said gel plug cartridge comprising a plurality of sections that are manually separable along predetermined separation lines, an internal cavity extending through all sections and filled with a gel in which is embedded a preselected mixture of solutes to be used as a standard, and, for any single section once separated from said cartridge, means for urging gel from said section out of said section and into a well of a slab gel.

### Claim 2 (depends on 1)

The gel plug cartridge of claim 1 wherein said plurality of sections are arranged linearly along said cartridge.

### Claim 3 (depends on 2)

The gel plug cartridge of claim 2 wherein said gel plug cartridge comprises a continuous molded piece with score lines therein separating said sections.

### Claim 4 (depends on 2)

The gel plug cartridge of claim 2 wherein said gel plug cartridge comprises a continuous molded piece formed in upper and lower halves, each half containing score lines separating said sections.

### Claim 5 (depends on 1)

The gel plug cartridge of claim 1 wherein said means for urging is a flat blade affixed to said section and manually separable therefrom along a score line.

### Claim 6 (depends on 1)

The gel plug cartridge of claim 1 further comprising, for any single section, a handle capable of being grasped manually.

### Claim 7 (depends on 1)

The gel plug cartridge of claim 1 further comprising ports at opposing ends of said internal cavity and end plugs sized to fit inside said ports and thereby seal said internal cavity.