# US 7,408,055 B2 · Promoter molecules for use in plants

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- Number: US 7,408,055 B2
- Document ID: US7408055B2
- Status: Granted
- Kind: B2
- Application number: 11/352,157
- Filed: 2006-02-10
- Published: 2006-08-10
- Granted: 2008-08-05
- Earliest claimed: 2005-02-10
- Assignees: Monsanto Technology LLC
- Inventors: Santanu Dasgupta; Wei Wu; Brendan S. Hinchey
- Web page: https://patentgrep.com/patent/US7408055B2

## CPC

- C12N 15/8222: {Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention provides DNA molecules isolated from Oryza sativa plants and useful for expressing transgenes in plants. The present invention also provides expression constructs containing the DNA molecules useful for expressing transgenes in plants. The present invention also provides transgenic plants and seeds containing the DNA molecules useful for expressing transgenes in plants.

## Claims

### Claim 1

An isolated DNA molecule having promoter function and comprising
(a) at least 50 contiguous bases from SEQ ID NO:6 comprising rice CUT1 promoter activity;
(b) a sequence comprising SEQ ID NO:6; or
(c) a fragment of SEQ ID NO:6 comprising rice CUT1 promoter activity,
wherein the DNA molecule having promoter function is operably linked to a heterologous transcribable DNA molecule.

### Claim 2 (depends on 1)

A recombinant DNA expression cassette having a promoter comprising the DNA molecule of claim 1.

### Claim 3 (depends on 2)

A transgenic plant having in its genome a recombinant DNA expression cassette of claim 2.

### Claim 4 (depends on 2, 3)

A seed of a transgenic plant of claim 3, wherein the seed comprises the expression cassette of claim 2.

### Claim 5 (depends on 2, 4)

The seed of claim 4 wherein said transgenic plant is selected from the group consisting of corn, soybean, cotton wheat, rice and canola, and wherein the seed comprises the expression cassette of claim 2.

### Claim 6 (depends on 1)

A cell transformed with the DNA molecule of claim 1.

### Claim 7 (depends on 1)

The isolated DNA molecule of claim 1, wherein the DNA molecule comprises SEQ ID NO:6.

### Claim 8 (depends on 3)

The transgenic plant of claim 3, wherein the DNA molecule comprises SEQ ID NO:6.

### Claim 9 (depends on 4)

The seed of claim 4, wherein the DNA molecule comprises SEQ ID NO:6.

### Claim 10 (depends on 6)

The cell of claim 6, wherein the DNA molecule comprises SEQ ID NO:6.

### Claim 11 (depends on 1)

The isolated DNA molecule of claim 1, wherein the DNA molecule comprises at least 50 contiguous bases from SEQ ID NO:6 comprising rice CUT1 promoter activity.

### Claim 12 (depends on 1)

The isolated DNA molecule of claim 1, wherein the DNA molecule comprises a fragment of SEQ ID NO:6 comprising rice CUT1 promoter activity.

### Claim 13 (depends on 2)

The recombinant DNA expression cassette of claim 2, wherein the wherein the DNA molecule comprises SEQ ID NO:6.

### Claim 14 (depends on 2)

The recombinant DNA expression cassette of claim 2, wherein the wherein the DNA molecule comprises a fragment of SEQ ID NO:6 comprising rice CUT1 promoter activity.

### Claim 15 (depends on 3)

The transgenic plant of claim 3, wherein the DNA molecule comprises at least 50 contiguous bases from SEQ ID NO:6 comprising rice CUT1 promoter activity.

### Claim 16 (depends on 3)

The transgenic plant of claim 3, wherein the DNA molecule comprises a fragment of SEQ ID NO:6 comprising rice CUT1 promoter activity.

### Claim 17 (depends on 4)

The seed of claim 4, wherein the DNA molecule comprises at least 50 contiguous bases from SEQ ID NO:6 comprising rice CUT1 promoter activity.

### Claim 18 (depends on 4)

The seed of claim 4, wherein the DNA molecule comprises a fragment of SEQ ID NO:6 comprising rice CUT1 promoter activity.

### Claim 19 (depends on 6)

The cell of claim 6, wherein the DNA molecule comprises at least 50 contiguous bases from SEQ ID NO:6 comprising rice CUT1 promoter activity.

### Claim 20 (depends on 6)

The cell of claim 6, wherein the DNA molecule comprises a fragment of SEQ ID NO:6 comprising rice CUT1 promoter activity.