# US 7,408,048 B2 · Mammalian grainyhead transcription factors

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- Number: US 7,408,048 B2
- Document ID: US7408048B2
- Status: Granted
- Kind: B2
- Application number: 10/524,619
- Filed: 2003-08-08
- Published: 2006-07-20
- Granted: 2008-08-05
- Earliest claimed: 2002-08-09
- Assignees: Melbourne Health
- Inventors: Stephen Jane; Tomasz Wilanowski; Stephen Ting
- Web page: https://patentgrep.com/patent/US7408048B2

## CPC

- A01K 67/0276: Knock-out vertebrates
- C07K 14/4702: {Regulators; Modulating activity}
- A01K 2217/075: inducing loss of function, i.e. knock out
- A01K 2227/105: Murine
- A01K 2267/0306: Animal model for genetic diseases
- C12N 2830/008: cell type or tissue specific enhancer/promoter combination
- A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention provides mammalian homologs of the Drosophila Grainyhead (GRH) transcription factor, call MGR and means of identifying such proteins and their genes. Nucleic acid and protein sequences are provided for human and mouse MGR transcription factors. In addition mammalian isoforms of MGR including human MGR p49, human p70, mouse p70, mouse MGR p61, and human and mouse homologs of MGR, brother of mgr (BOM) and sister of mgr (SOM), are disclosed. Antibodies to and methods of using these identified MGR, BOM and SOM transcription factors are also provided. The present invention further provides medical assessment systems including drug evaluation systems comprising genetically modified animals.

## Claims

### Claim 1

An isolated nucleic acid molecule comprising a sequence of nucleotides encoding a mammalian transcription factor comprising the amino acid sequence set forth in SEQ ID NO: 8 or an amino acid sequence having at least 95% identity thereto.

### Claim 2 (depends on 1)

The isolated nucleic acid molecule of claim 1 wherein the molecule has a nucleotide sequence selected from the group consisting of: SEQ ID NO: 7, SEQ ID NO: 15, and a nucleotide sequence which hybridizes to the full-length, complementary sequences of SEQ ID NO: 7 or SEQ ID NO:15 under conditions of 0.1×SSC, 0.1% w/v SDS at 65° C.

### Claim 3 (depends on 1)

The isolated nucleic acid molecule of claim 1 encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 8 and SEQ ID NO: 16.

### Claim 4 (depends on 1)

The isolated nucleic acid molecule of claim 1 comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 7 and SEQ ID NO: 15.

### Claim 5 (depends on 1)

The isolated nucleic acid molecule of claim 1 comprising the nucleotide sequence set forth in SEQ ID NO: 7.

### Claim 6 (depends on 1)

The isolated nucleic acid molecule of claim 1 comprising the nucleotide sequence set forth in SEQ ID NO: 15.