# US 7,408,047 B1 · Fibroblast growth factor-like polypeptides

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 7,408,047 B1
- Document ID: US7408047B1
- Status: Granted
- Kind: B1
- Application number: 09/391,861
- Filed: 1999-09-07
- Granted: 2008-08-05
- Earliest claimed: 1999-09-07
- Assignees: Amgen Inc.
- Inventors: Arlen Read Thomason; Benxian Liu
- Web page: https://patentgrep.com/patent/US7408047B1

## CPC

- C07K 14/50: Fibroblast growth factor [FGF]
- A61P 3/04: Anorexiants; Antiobesity agents
- A61P 3/10: for hyperglycaemia, e.g. antidiabetics
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention provides novel Fibroblast Growth Factor-like (FGF-like) polypeptides and nucleic acid molecules encoding the same. The invention also provides vectors, host cells, antibodies and methods for producing FGF-like polypeptides. Also provided for are methods for the diagnosis and treatment of diseases associated with FGF-like polypeptides.

## Claims

### Claim 1

An isolated nucleic acid molecule comprising a nucleotide sequence:
(a) as set forth in either SEQ ID NO: 1 or SEQ ID NO: 3;
(b) of the DNA insert in ATCC Deposit No. PTA-626; or
(c) encoding an FGF-like polypeptide as set forth in either SEQ ID NO: 2 or SEQ ID NO: 4.

### Claim 2 (depends on 1)

A recombinant host cell comprising the nucleic acid molecule of claims 1.

### Claim 3 (depends on 2)

The recombinant host cell of claim 2 which is a eukaryotic cell.

### Claim 4 (depends on 2)

The recombinant host cell of claim 2 which is a prokaryotic cell.

### Claim 5 (depends on 1)

A process of producing a polypeptide encoded by the nucleic acid molecule of claim 1, comprising culturing a recombinant host cell comprising the nucleic acid molecule of claim 1 under suitable conditions to express the polypeptide.

### Claim 6 (depends on 5)

The process of claim 5, wherein the nucleic acid molecule comprises promoter DNA other than the promoter DNA for a native FGF-like gene operatively linked to the nucleic acid molecule.

### Claim 7 (depends on 1)

A vector comprising the nucleic acid molecule of claim 1.

### Claim 8 (depends on 7)

A recombinant host cell comprising the vector of claim 7.

### Claim 9 (depends on 8)

The recombinant host cell of claim 8 which is a eukaryotic cell.

### Claim 10 (depends on 8)

The recombinant host cell of claim 8 which is a prokaryotic cell.

### Claim 11 (depends on 2)

A process for determining whether a compound inhibits FGF-like polypeptide production comprising exposing a cell according to claim 2 to the compound, and measuring FGF-like polypeptide production in said cell.

### Claim 12 (depends on 5)

The process of claim 5, further comprising recovering the polypeptide from the culture.

### Claim 13 (depends on 1)

A process of producing a polypeptide encoded by the nucleic acid molecule of claim 1, comprising culturing a recombinant host cell comprising a vector comprising the nucleic acid molecule of claim 1 under suitable conditions to express the polypeptide.

### Claim 14 (depends on 13)

The process of claim 13, further comprising recovering the polypeptide from the culture.

### Claim 15

An isolated nucleic acid molecule comprising a nucleotide sequence encoding an FGF-like polypeptide as set forth in either SEQ ID NO: 5 or SEQ ID NO: 6.