# US 7,408,045 B2 · Adsorbent of high-mobility-group protein and body fluid-purification column

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- Number: US 7,408,045 B2
- Document ID: US7408045B2
- Status: Granted
- Kind: B2
- Application number: 11/490,095
- Filed: 2006-07-21
- Published: 2006-11-16
- Granted: 2008-08-05
- Earliest claimed: 2000-04-05
- Assignees: Toray Industries, Inc.
- Inventors: Ikuro Maruyama; Nobuo Ida; Sanae Masuko
- Web page: https://patentgrep.com/patent/US7408045B2

## CPC

- B01J 20/26: Synthetic macromolecular compounds
- B01J 20/261: {obtained by reactions only involving carbon to carbon unsaturated bonds (macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds per se C08F)}
- B01J 20/262: {obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation (macromolecular compounds obtained otherwise than by reactions only involving unsaturated carbon-to-carbon bonds per se C08G)}
- B01J 20/267: {Cross-linked polymers}
- B01J 20/28016: {Particle form}
- B01J 20/28023: {Fibres or filaments (fibres or filaments in the form of membranes B01J20/28038; B01J20/28007 takes precedence)}
- B01J 20/321: {consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds}
- B01J 20/3212: {consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds}
- B01J 20/3217: {Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond}
- B01J 20/3219: {involving a particular spacer or linking group, e.g. for attaching an active group}
- B01J 20/3225: {involving a post-treatment of the coated or impregnated product}
- B01J 20/3246: {having a well defined chemical structure}
- B01J 20/3248: {the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such}
- B01J 20/3251: {comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur}
- B01J 20/3274: {Proteins, nucleic acids, polysaccharides, antibodies or antigens}
- B01J 39/17: containing also inorganic materials, e.g. inert material coated with an ion-exchange resin
- B01J 39/18: Macromolecular compounds (B01J39/17 takes precedence)
- B01J 41/10: Inorganic material
- B01J 41/12: Macromolecular compounds
- A61M 1/3679: {by absorption (A61M1/3675 takes precedence)}
- B01J 2220/58: Use in a single column
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- A61M: DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY (introducing media into or onto the bodies of animals A61D7/00; means for inserting tampons A61F13/26; devices for administering food or medicines orally A61J; containers for collecting, storing or administering blood or medical fluids A61J1/05); DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY (surgery A61B; chemical aspects of surgical articles A61L); DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR {(Electrotherapy, e.g. producing anaesthesia by the use of alternating or intermittent currents A61N1/36021)}

## Abstract

An adsorbent of high-mobility-group proteins (HMG protein) which can remove HMG protein in body fluid is disclosed. The adsorbent according to the present invention has a water-insoluble carrier on which (a) substance(s) having (a) hydrogen-bondable functional group(s) and/or (a) hydrophobic functional group(s) is(are) immobilized.

## Claims

### Claim 1

A method for adsorbing high-mobility-group proteins in body fluid, comprising:
contacting an adsorbent with body fluid so as to adsorb said high-mobility-group proteins in said body fluid to said adsorbent;
wherein said adsorbent comprises a water-insoluble carrier on which a substance(s) having a hydrogen-bondable functional group(s) and/or a hydrophobic functional group is(are) immobilized, and
wherein said adsorbent has a rate of adsorption for high-mobility-group proteins of not less than 50%, and has a rate of adsorption for serum albumin of not more than 20% and wherein said body fluid is blood.

### Claim 2 (depends on 1)

The method according to claim 1, which is carried out by using a body fluid-purification column for removing high-mobility-group proteins, said body fluid-purification column comprising a column and said absorbent is packed in said column.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the body fluid is obtained from a patient that has sepsis.

### Claim 4 (depends on 3)

The method according to claim 3, which is carried out by using a body fluid-purification column for removing high-mobility-group proteins, said body fluid- purification column comprising a column and said absorbent is packed in said column, together with a body fluid-purification column(s) which adsorbs a substance(s) originating from bacteria.

### Claim 5 (depends on 2)

The method according to claim 2, wherein extracorporcal circulation of whole blood is attained by said body fluid-purification column step.

### Claim 6 (depends on 1)

The method according to claim 1, wherein said hydrogen-bondable functional group(s) is(are) a cationic functional group(s).

### Claim 7 (depends on 6)

The method according to claim 6, wherein said cationic functional group(s) is(are) at least one selected from the group consisting of a primary amino group, secondary amino group, tertiary amino group, imino group and/or quaternary ammonium group.

### Claim 8 (depends on 6)

The method according to claim 6, wherein said cationic functional group(s) is(are) represented by the following formula (I):
wherein cach of R1,R2and R3independently represents a hydrogen atom or a C1-C5alkyl group.

### Claim 9 (depends on 8)

The mcthod according to claim 8, wherein each of R1, R2and R3independently represents a hydrogen atom or a C1-C2alkyl group.

### Claim 10 (depends on 8)

The method according to claim 8, wherein each of R1, R2and R3independently represents a methyl group.

### Claim 11 (depends on 1)

The method according to claim 1, wherein said hydrogen-bondable functional group(s) is(are) an anionic functional group(s).

### Claim 12 (depends on 11)

The method according to claim 11, wherein said hydrophobic functional group(s) is(are) at least one selected from the group consisting of a carboxyl group, sulfate group, sulfonic acid group and phosphate group.

### Claim 13 (depends on 1)

The method according to claim 1, wherein said hydrophobic functional group(s) is(are) an alkyl group(s) having not less than 6carbon atoms or an aromatic group(s).

### Claim 14 (depends on 1)

The method according to claim 1, wherein said substance(s) having the hydrogen-bondable functional group(s) and/or a hydrophobic functional group(s) is(are) a peptide(s) or an amino acid(s).

### Claim 15 (depends on 7)

The method according to claim 7, wherein said substance(s) having the hydrogen-bondable functional group(s) is(are) a peptide(s) or an amino acid(s) having an amino group(s) in its(their) side chain(s).

### Claim 16 (depends on 15)

The method according, to claim 15, wherein said substance having the hydrogen-bondable functional group(s) is(are) polylysine.

### Claim 17 (depends on 11)

The method according to claim 11, wherein a polysaccharide(s) having a sulfate group(s) is(are) immobilized.

### Claim 18 (depends on 17)

The method according to claim 17, wherein said polysaccharide is heparin or dextran sulfate, or a derivative thereof.

### Claim 19 (depends on 1)

The method according to claim 1, wherein said adsorbent comprises the water-insoluble carrier on which an antibody(ies) to said high-mobility-group proteins is(are) immobilized.

### Claim 20 (depends on 19)

The method according to claim 19, wherein said adsorbent has a rate of adsorption for high-mobility-group proteins of not less than 50%, and has a rate of adsorption for serum albumin of not more than 20%.

### Claim 21 (depends on 1, 19)

The method according to claim 1 or 19, wherein said water-insoluble carrier is in the form of fibers.

### Claim 22 (depends on 1, 19)

The method according to claim 1 or 19, wherein said water-insoluble carrier is in the form of beads.