# US 7,407,791 B2 · Production of secreted polypeptides

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- Number: US 7,407,791 B2
- Document ID: US7407791B2
- Status: Granted
- Kind: B2
- Application number: 10/636,682
- Filed: 2003-08-06
- Published: 2005-04-28
- Granted: 2008-08-05
- Earliest claimed: 1999-12-21
- Assignees: Genencor International, Inc.
- Inventors: Maria R. Diaz-Torres
- Web page: https://patentgrep.com/patent/US7407791B2

## CPC

- C12N 9/2417: {from microbiological source}
- C12N 9/54: bacteria being Bacillus
- C12N 15/52: Genes encoding for enzymes or proenzymes
- C12N 15/75: for Bacillus
- C12P 21/02: having a known sequence of two or more amino acids, e.g. glutathione
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}

## Abstract

Described herein are methods and compositions for the production and secretion of polypeptides. Included herein is the use of interrupting peptide transport activity for an increase in polypeptide production and/or secretion.

## Claims

### Claim 1

An isolated Bacillus cell comprising a peptide transport operon, wherein said operon is a Bacillus dciA operon or opp operon, and wherein said operon has been mutated by making inactivating deletions or insertions in a gene in the operon such that said cell has increased polypeptide secretion.

### Claim 2 (depends on 1)

The cell of claim 1 wherein the dciAE gene or the dciAA gene has been partially or completely deleted.

### Claim 3

An isolated Escherichia coli cell comprising a peptide transport operon, wherein said operon is an Escherichia coli dpp operon or opp operon, and wherein said operon has been mutated by making inactivating deletions or insertions in a gene in the operon such that said cell has increased polypeptide secretion.