Recombinant double-stranded RNA phages and uses thereof
AssigneeUniversity of Maryland Biotechnology Institute
InventorDavid Hone
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Abstract
The present invention provides recombinant double stranded RNA phages (rdsRP) that express dsRNA-encoded genes in eukaryote cells. Recombinant dsRNA phages are useful for the expression of dsRNA expression cassettes encoding passenger genes, such as, but not restricted to, vaccine antigens, bioactive proteins, immunoregulatory proteins, antisense RNAs, and catalytic RNAs in eukaryotic cells or tissues. Methods are provided to deliver a recombinant dsRNA phage to eukaryotic cells and tissues, either by direct administration, formulated in lipid or polylactidecoglycolide, or by utilizing a bacterial vaccine vector.
Claims32
A recombinant double stranded RNA phage (rdsRP) comprising a double stranded RNA eukaryotic expression cassette for expression in eukaryotic cells, the rdsRP comprising:
- at least one genomic segment of a double stranded RNA phage (dsgP) and an internal ribosome entry site (IRES) nucleotide sequence incorporated into the at least one genomic segment of the dsRP.
Dependencies are inferred from the claim text.