# US 7,407,761 B2 · Methods for assaying expression of novel galanin receptors

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- Number: US 7,407,761 B2
- Document ID: US7407761B2
- Status: Granted
- Kind: B2
- Application number: 11/407,004
- Filed: 2006-04-18
- Published: 2006-10-19
- Granted: 2008-08-05
- Earliest claimed: 1996-07-19
- Assignees: National Research Council of Canada
- Inventors: Shi-Hsiang Shen; Sultan Ahmad; Claes Wahlestedt; Philippe Walker
- Web page: https://patentgrep.com/patent/US7407761B2

## CPC

- G01N 33/74: involving hormones {or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors}
- C07K 14/72: for hormones {(for neuromediators C07K14/70571)}
- G01N 2500/00: Screening for compounds of potential therapeutic value
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The present invention is directed to methods for assaying a test compound's ability to alter the expression of novel receptor for galanin which has been designated as galanin receptor 2. The invention also encompasses both the receptor protein as well as nucleic acids encoding the protein. In addition, the present invention is directed to additional methods and compositions which rely upon either GAL-R2 proteins or nucleic acids.

## Claims

### Claim 1

A method for assaying a test compound for its ability to alter the expression of a protein comprising:
growing cells expressing the protein but substantially free of other galanin receptors in the presence of the test compound;
collecting the cells; and
comparing the expression of the protein in the cells exposed to the test compound with control cells grown under essentially identical conditions but not exposed to the test compound, wherein a difference in expression indicates the ability of the test compound to alter expression of the protein, wherein the protein comprises SEQ ID NO:4.

### Claim 2 (depends on 1)

A method of claim 1 wherein the protein expression is determined by a receptor binding assay.

### Claim 3

A method for assaying a test compound for its ability to alter the expression of a protein comprising:
growing cells expressing the GALR2 protein but substantially free of other galanin receptors in the presence of the test compound;
collecting the cells; and
comparing the expression of the protein in the cells exposed to the test compound with control cells grown under essentially identical conditions but not exposed to the test compound, wherein a difference in expression indicates the ability of the test compound to alter expression of the protein, wherein the cells expressing the protein are cells transformed with an expression vector comprising a polynucleotide sequence comprising SEQ ID NO:1, wherein said vector encodes for said protein.

### Claim 4 (depends on 3)

A method of claim 3 wherein the test compound is an oligonucleotide at least 15 nucleotides in length and comprising a sequence complementary to SEQ ID NO: 1.

### Claim 5

A method for assaying a test compound for its ability to alter the expression of a protein comprising:
growing cells expressing the protein but substantially free of other galanin receptors, wherein the cells expressing the protein are cells transformed with an expression vector comprising a polynucleotide sequence encoding the protein, wherein the protein comprises SEQ ID NO:4, in the presence of the test compound;
collecting the cells; and
comparing expression of the protein in the cells exposed to the test compound with control cells grown under essentially identical conditions but not exposed to the test compound, wherein a difference in expression indicates the ability of the test compound to alter expression of the protein.

### Claim 6 (depends on 5)

A method of claim 5 wherein the polynucleotide sequence comprises SEQ ID NO:3.

### Claim 7 (depends on 5)

A method of claim 5 wherein the test compound is an oligonucleotide at least 15 nucleotides in length and comprising a sequence complementary to SEQ ID NO:3.