# US 7,407,652 B2 · Probiotic system for aquaculture

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- Number: US 7,407,652 B2
- Document ID: US7407652B2
- Status: Granted
- Kind: B2
- Application number: 11/144,929
- Filed: 2005-06-03
- Published: 2006-12-07
- Granted: 2008-08-05
- Earliest claimed: 2005-06-03
- Assignees: AquaTechnics
- Inventors: Ralph A. Elston; Arthur Gee; Karen L. Humphrey
- Web page: https://patentgrep.com/patent/US7407652B2

## CPC

- A61P 31/00: Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- C12N 1/205: Bacterial isolates
- A01N 63/20: Bacteria; Substances produced thereby or obtained therefrom
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- Y10S 435/822: using bacteria or actinomycetales
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

Probiotic bacterial strains are provided which inhibit or prevent growth or pathogenic bacteria in marine organisms. Also provided are methods of culturing marine organisms using the probiotic bacteria.

## Claims

### Claim 1

A biologically pure bacterial strain of bacteria Pseudoalteromonas P02-1 deposited as ATCC PTA-6677.

### Claim 2 (depends on 1)

A method of inhibiting a pathogenic bacterial infection in marine organisms, comprising bathing a stock of marine organisms in a culture containing the bacterial strain of claim 1.

### Claim 3 (depends on 2)

The method of claim 2 wherein said marine organisms are mollusks.

### Claim 4 (depends on 2)

The method of claim 2, wherein the marine organisms are cultured food fish, including but not limited to salmon, trout, grouper, tuna fishes and all marine species whose early life stages may be reared under controlled conditions in seawater hatcheries and nurseries.

### Claim 5 (depends on 2)

The method of claim 2 wherein said marine organisms are in the nauplii, larval, post-larval, or juvenile stage, or are adults or are brood stock.

### Claim 6 (depends on 1)

A method of preventing growth of pathogenic bacteria by treatment of marine organism rearing tanks with bacterial strain of claim 1 prior to the introduction of marine organisms to the tank.

### Claim 7 (depends on 1)

A composition comprising the bacterial strain of claim 1 and a culture selected from the group consisting of unicellular algae and multi-cellular algae.

### Claim 8

A biologically pure bacterial strain of bacteria Pseudoalteromonas P02-45 deposited as ATCC PTA-6678.

### Claim 9 (depends on 8)

A method of inhibiting a pathogenic bacterial infection in marine organisms, comprising bathing a stock of marine organisms in a culture containing the bacterial strain of claim 8.

### Claim 10 (depends on 9)

The method of claim 9 wherein said marine organisms are mollusks.

### Claim 11 (depends on 9)

The method of claim 9, wherein the marine organisms are cultured food fish, including but not limited to salmon, trout, grouper, tuna fishes and all species whose early life stages may be reared under controlled conditions in seawater hatcheries and nurseries.

### Claim 12 (depends on 8)

A method of preventing growth of pathogenic bacteria by treatment of marine organism rearing tanks with bacterial strain of claim 8 prior to the introduction of marine organisms to the tank.

### Claim 13 (depends on 8)

A composition comprising the bacterial strain of claim 8 and a culture selected from the group consisting of unicellular algae and multi-cellular algae.

### Claim 14

A composition comprising a mixture of biologically pure bacterial strains Pseudoalteromonas P02-1 deposited as ATCC PTA-6677 and Pseudoalteromonas P02-45 deposited as ATCC PTA-6678 wherein said mixture contains between 1% and 99% colony forming units of strain Pseudoalteromonas P02-1 deposited as ATCC PTA-6677 and between 1% and 99% colony forming units of strain P02-45 deposited as ATCC PTA-6678.

### Claim 15 (depends on 14)

A method of inhibiting a pathogenic bacterial infection in marine organisms, comprising bathing a stock of said marine organisms in a culture containing the composition of claim 14.

### Claim 16 (depends on 15)

The method of claim 15 wherein said marine organisms are mollusks.

### Claim 17 (depends on 15)

The method of claim 15, wherein the marine organisms are cultured food fish, including but not limited to salmon, trout, grouper, tuna fishes and all species whose early life stages may be reared under controlled conditions in seawater hatcheries and nurseries.

### Claim 18

A method of preventing growth of pathogenic bacteria in commercial and residential salt water aquariums wherein the aquarium is treated with bacterial strain Pseudoalteromonas P02-1 deposited as ATCC PTA-6677, P02-45 deposited as ATCC PTA-6678, or a mixture thereof.