System, method, and computer product for detection instrument calibration
AssigneeAffymetrix, Inc.
InventorChristopher Miles
Life of this patent
Estimating the term…
Abstract
In one embodiment, a method of determining a drift value is described that includes performing one or more X-axis translations of an excitation beam over a probe array; measuring light responsive to the excitation beam from at least two positional reference elements associated with the probe array for the X-axis translations; calculating a distance value for the X-axis translation using a positional relationship of a known location associated with each of the positional reference elements and positions of the positional reference elements from the measured light; and determining a drift value using a difference between the calculated distance value and an expected distance value.
Claims30
A method of determining a drift value, comprising:
- performing one or more X-axis translations of an excitation beam over a probe array;
- measuring light responsive to the excitation beam from at least two positional reference elements associated with the probe array for the X-axis translations;
- calculating a distance value for the X-axis translation using a positional relationship of a known location associated with each of the positional reference elements and positions of the positional reference elements from the measured light;
- and
- determining a drift value using a difference between the calculated distance value and an expected distance value.
A system for determining a drift value, comprising:
- a translation element that performs one or more X-axis translations of an excitation beam over a probe array;
- one or more detectors that measure light responsive to the excitation beam from at least two positional reference elements associated with the probe array for the X-axis translations;
- a software application stored for execution is system memory of a computer that performs the method of:
- calculating a distance value associated with the X-axis translation;
- determining a drift value using a difference between the calculated distance value and an expected distance value.
A method of correcting X-axis error in a probe array image, comprising:
- performing one or more X-axis translations of an excitation beam over a probe array;
- measuring light responsive to the excitation beam from at least two positional reference elements associated with the probe array for the X-axis translations;
- calculating a distance value for the X-axis translation using a positional relationship of a known location associated with each of the positional reference elements and positions of the positional reference elements from the measured light;
- determining a drift value using a difference between the calculated distance value and an expected distance value;
- and
- applying the drift value to a plurality of pixels of an image of a plurality of probe features acquired from the probe array prior to grid placement.
A probe array scanner for correcting X-axis error in a probe array image, comprising:
- a probe array scanner that comprises;
- a translation element that performs one or more X-axis translations of an excitation beam over a probe array;
- one or more detectors that measure light responsive to the excitation beam from at least two positional reference elements associated with the probe array for the X-axis translations;
- and
- a software application stored for execution in system memory of a computer that performs the method of:
- calculating a distance value for the X-axis translation using a positional relationship of a known location associated with each of the positional reference elements and positions of the positional reference elements from the measured light;
- determining a drift value using a difference between the calculated distance value and an expected distance value;
- and applying the drift value to a plurality of pixels of image of a plurality of probe features acquired from the probe array prior to grid placement.
Dependencies are inferred from the claim text.