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AssigneeOc Oerlikon Balzers AG
InventorsJorg Kraus, Johannes Edlinger, Max Wiki, Heidi Thome-Forster, Claus Heine-Kempkens, Bernd Maisenhoelder, Martin Kaspar
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A sample substrate adapted for use with fluorescence excitation light with a first wavelength. A reflector is disposed on a base. The reflector includes a reflecting multilayer interference coating with at least two layers. Not all of the layers L fulfill a quarterwave condition: dL·nL=(2N+1)·¼ wherein dL is a physical thickness of layer L, nL is an index of refraction of layer L at the first wavelength, N is an integer equal to or greater than zero and 1 is the first wavelength. Thicknesses of the layers ensure that any fluorescent sample material disposed on top of said multilayer interference coating would be located near an antinode of a standing wave formed by the excitation light with the first wavelength incident on said substrate.
A sample substrate adapted for use with fluorescence excitation light with a first wavelength and a second wavelength, comprising a base and a reflector, the reflector comprising a reflecting multilayer interference coating comprising a first layer system including multiple layers and an intermediate layer system including multiple layers, wherein the first layer system reflects the excitation light with the first wavelength and transmits the excitation light with the second wavelength, and thicknesses of the layers of the first layer system ensure that any fluorescent sample material deposited on top of said multilayer interference coating is located near an antinode of a standing wave formed by the excitation light with the first wavelength incident on said substrate, and wherein the intermediate layer system reflects the excitation light with the second wavelength, and a thickness of at least one layer of the intermediate layer system ensures that the sample material deposited on top of said multilayer interference coating is located near an antinode of a standing wave formed by the excitation light with the second wavelength incident on said substrate, and further wherein the reflecting multilayer interference coating causes the antinodes to be located at substantially the same position.
Dependencies are inferred from the claim text.