V-like domain binding molecules and polynucleotides encoding therefor
AssigneeDiatech Pty Ltd.
InventorsMaria Galanis, Peter John Hudson, Robert Alexander Irving, Stewart Douglas Nuttall
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Abstract
The present invention relates to binding moieties comprising at least one monomeric V-like domain (VLD) derived from a non-antibody ligand, the at least one monomeric V-like domain being characterised in that at least one CDR loop structure or part thereof is modified or replaced such that the solubility of the modified VLD is improved when compared with the unmodified VLD.
Claims20
An isolated polynucleotide encoding a monomeric non-antibody ligand V-like domain (VLD) comprising within the VLD at least one CDR loop structure or part thereof that is modified or replaced such that
- (i) the size of the CDR loop structure or part thereof is increased by at least one amino acid residue when compared with the corresponding CDR loop structure or part thereof in an unmodified VLD;
- and/or
- (ii) the modification or replacement results in formation of a disuiphide bond within or between one or more of the CDR loop structures, wherein the CDR loop structure is a surface polypeptide loop structure corresponding to a complementarity determining region of an antibody V-domain, and wherein the non-antibody ligand is selected from the group consisting of CTLA-4, CD28 and ICOS.
Dependencies are inferred from the claim text.